Asymmetric Syncytial Expression of GLUT9 Splice Variants in Human Term Placenta and Alterations in Diabetic Pregnancies
Asymmetric Syncytial Expression of GLUT9 Splice Variants in Human Term Placenta and Alterations in Diabetic Pregnancies
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DOI：
10.1177/1933719110380276
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Moley, Kelle H.
中科院分区：
文献类型：
--
作者：
Bibee, Kristin P.;Illsley, Nicholas P.;Moley, Kelle H.
由于胎儿糖异生的缺失，葡萄糖从母体到胎盘胎儿侧的转运对胎儿的生长发育至关重要。人类GLUT9是转运蛋白家族的新成员，有2种同种异构体存在。本研究研究了这些异构体在正常人和糖尿病人足月胎盘中的定位和相对表达水平。从正常妊娠和合并糖尿病的孕妇中收集胎盘样本（White分类A1、A2和B）。使用不同同种异构体的特异性抗体检测表达。两种形式的蛋白都在合胞滋养层细胞中表达。亚细胞分离显示，GLUT9a在基膜上不对称表达，而GLUT9b则定位于微绒毛膜。这两种异构体的表达在糖尿病妊娠的胎盘组织中显著增加。胎盘中GLUT9表达的改变可能在与糖尿病合并妊娠相关的胎儿病理生理中起作用。
Glucose transport from the maternal to fetal side of the placenta is critical for fetal growth and development due to the absence of fetal gluconeogenesis. Human GLUT9, existing as 2 isoforms, is a novel member of the transporter family. This study investigated the localization and relative expression levels of these isoforms in the human term placenta from both control and diabetic patients. Placenta samples were collected from normal pregnancies and those complicated by maternal diabetes (White classifications A1, A2, and B). Antibodies specific for the different isoforms were used to detect expression. Both forms of the protein are expressed in syncytiotrophoblast cells. Subcellular fractionation revealed an asymmetrical expression pattern with GLUT9a on basal membranes, whereas GLUT9b localizes to microvillus membranes. Expression of both isoforms is significantly increased in placental tissue from diabetic pregnancies. Altered expression of GLUT9 in the placenta may play a role in the fetal pathophysiology associated with diabetes-complicated pregnancies.