A global profile of germline gene expression in C-elegans
A global profile of germline gene expression in C-elegans
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DOI：
10.1016/s1097-2765(00)00059-9
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发表时间：
2000-09-01
期刊：
影响因子：
16
通讯作者：
Kim, SK
中科院分区：
文献类型：
--
作者：
Reinke, V;Smith, HE;Kim, SK
我们使用DNA微阵列来分析线虫种系中的基因表达模式，并鉴定了1416个种系丰富的转录本，这些转录本定义了三个组。精子丰富组含有异常大量的蛋白激酶和磷酸酶。丰富卵母细胞组可能包括胚胎信号通路的新成分。生殖系固有组是指在只产生精子或只产生卵母细胞的生殖系中表达相似的基因，它包含一系列可能对干细胞增殖至关重要的Piwi相关基因。最后，对生殖系转录物的染色体位置的检查发现，X染色体上几乎没有精子丰富的基因和生殖系固有的基因，但丰富的卵母细胞基因却没有。
We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new components of embryonic signaling pathways. The germline-intrinsic group, defined as genes expressed similarly in germlines making only sperm or only oocytes, contains a family of piwi-related genes that may be important for stem cell proliferation. Finally, examination of the chromosomal location of germline transcripts revealed that sperm-enriched and germline-intrinsic genes are nearly absent from the X chromosome, but oocyte-enriched genes are not.