Engineering the human gut commensal Bacteroides thetaiotaomicron with synthetic biology
Engineering the human gut commensal Bacteroides thetaiotaomicron with synthetic biology
复制标题
利用合成生物学改造人类肠道共生多形拟杆菌
DOI：
10.1016/j.cbpa.2022.102178
复制
发表时间：
2022
影响因子：
7.8
通讯作者：
Lu, Timothy K.
中科院分区：
文献类型：
--
作者：
Lai, Yong;Hayashi, Naoki;Lu, Timothy K.
微生物组在健康和疾病中的作用正吸引着寻求将微生物用于诊断和治疗应用的研究人员的注意。合成生物学的最新进展可能使宿主-微生物群相互作用的解剖成为可能。在人类肠道中占主导地位的稳定共生细菌拟杆菌（Bacteroides thetaiotaomicron）已经开发出了能够感知、计算、记忆和响应信号的复杂遗传电路。在这篇综述中，我们重点介绍了扩大b基因工具箱的最新进展。并展望了该物种在微生物组工程中的几种应用。我们对人类肠道相关细菌工程作为活治疗剂的挑战和未来机遇提供了我们的观点。
The role of the microbiome in health and disease is attracting the attention of researchers seeking to engineer microorganisms for diagnostic and therapeutic applications. Recent progress in synthetic biology may enable the dissection of host–microbiota interactions. Sophisticated genetic circuits that can sense, compute, memorize, and respond to signals have been developed for the stable commensal bacterium Bacteroides thetaiotaomicron, dominant in the human gut. In this review, we highlight recent advances in expanding the genetic toolkit forB. thetaiotaomicronand foresee several applications of this species for microbiome engineering. We provide our perspective on the challenges and future opportunities for the engineering of human gut-associated bacteria as living therapeutic agents.