Fast myosin heavy chain expression during the early and late embryonic stages of chicken skeletal muscle development.
Fast myosin heavy chain expression during the early and late embryonic stages of chicken skeletal muscle development.
复制标题
鸡骨骼肌发育早期和晚期胚胎阶段肌球蛋白重链的快速表达。
DOI：
10.1016/0012-1606(89)90100-0
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发表时间：
1989
影响因子：
2.7
通讯作者：
Crow,MT
中科院分区：
文献类型：
--
作者：
VanHorn,R;Crow,MT
鸡胚胎骨骼肌的发育可分为两个纤维特化时期--早期阶段，在此期间形成四肢的不同肌肉，并发生第一轮纤维特化;晚期或胎儿期，在此期间，这种先前建立的纤维模式广泛生长。后一个生长期依赖于功能性神经肌肉接触的建立和维持。正如已经描述的其他发育阶段，我们在这里表明，有不同的胚胎快速骨骼肌肌球蛋白重链（MHC）亚型在不同的胚胎时期的肌肉生长。这些亚型的鉴定是基于它们与各种快速MHC单克隆抗体的反应性差异以及它们不同的肽带型。胚胎晚期MHC亚型在卵内的积累与先前描述的胚胎发生过程中α-肌动蛋白和肌钙蛋白T亚型转换的时间过程相似。晚期胚胎亚型的出现被阻断慢性治疗与神经肌肉阻断剂，d-筒箭毒碱，和细胞培养的胚胎鸡骨骼肌分化的运动神经元的情况下，表达很少的晚期胚胎亚型，表明晚期胚胎亚型的表达是依赖于功能性神经肌肉的相互作用。这些不同的胚胎快速MHC亚型提供了重要的标志物监测肌肉的进展，通过其胚胎阶段和其相互作用与运动神经元。
The development of embryonic skeletal muscles in the chick can be divided into two periods of fiber specialization—an early one during which the different muscles of the limb are formed and an initial round of fiber specialization occurs and a late or fetal period during which there is extensive growth of this previously established fiber pattern. This latter period of growth is dependent on the establishment and maintenance of functional neuromuscular contacts. As has been described for other developmental stages, we show here that there are different embryonic fast skeletal muscle myosin heavy chain (MHC) isoforms expressed during the different embryonic periods of muscle growth. The identification of these isoforms was based on differences in their reactivity with various fast MHC monoclonal antibodies and on their different peptide banding patterns. Thein ovoaccumulation of the late embryonic MHC isoform pattern was similar to the time course of the previously described changes in α-actin and troponin T isotype switching during embryogenesis. The appearances of the late embryonic isoforms were blocked by chronic treatment with the neuromuscular blocking agent,d-tubocurarine, and cell cultures of embryonic chicken skeletal muscle which differentiated in the absence of motorneurons expressed little of the late embryonic isoform, indicating that the expression of the late embryonic isoform was dependent on functional nerve-muscle interactions. These different embryonic fast MHC isoforms provide important markers for monitoring the progression of muscle through its embryonic stages and its interaction with motorneurons.