Wireless optofluidic brain probes for chronic neuropharmacology and photostimulation
Wireless optofluidic brain probes for chronic neuropharmacology and photostimulation
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DOI：
10.1038/s41551-019-0432-1
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发表时间：
2019-08-01
影响因子：
28.1
通讯作者：
Jeong, Jae-Woong
中科院分区：
文献类型：
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作者：
Qazi, Raza;Gomez, Adrian M.;Jeong, Jae-Woong
体内神经药理学和光遗传学刺激均可用于解码神经回路，并可为脑疾病提供治疗策略。然而，目前的神经元接口阻碍了在清醒和自由行为的动物中的长期研究，因为它们在提供多种药物的同时和延长递送的能力方面受到限制，通常体积庞大且缺乏多功能性，并且采用定制控制系统，其对于输出模式、动物选择和目标脑回路的选择性不够通用。在这里，我们描述了智能手机控制的，微创的，软的光流体探针与可更换的插头样药物盒的慢性体内药理学和光遗传学与脑回路的选择性操纵。我们证明了使用探针通过可编程无线药物递送和光刺激控制小鼠的运动活动超过四周。由于它们能够在很长一段时间内将药物和光药理学反复输送到大脑中，这些探针可能有助于揭示神经精神疾病的基础。
Both in vivo neuropharmacology and optogenetic stimulation can be used to decode neural circuitry, and can provide therapeutic strategies for brain disorders. However, current neuronal interfaces hinder long-term studies in awake and freely behaving animals, as they are limited in their ability to provide simultaneous and prolonged delivery of multiple drugs, are often bulky and lack multifunctionality, and employ custom control systems with insufficiently versatile selectivity for output mode, animal selection and target brain circuits. Here, we describe smartphone-controlled, minimally invasive, soft optofluidic probes with replaceable plug-like drug cartridges for chronic in vivo pharmacology and optogenetics with selective manipulation of brain circuits. We demonstrate the use of the probes for the control of the locomotor activity of mice for over four weeks via programmable wireless drug delivery and photostimulation. Owing to their ability to deliver both drugs and photopharmacology into the brain repeatedly over long time periods, the probes may contribute to uncovering the basis of neuropsychiatric diseases.