Isolation of Mycobacterium tuberculosis Strains with a Silent Mutation in rpoB Leading to Potential Misassignment of Resistance Category
Isolation of Mycobacterium tuberculosis Strains with a Silent Mutation in rpoB Leading to Potential Misassignment of Resistance Category
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DOI：
10.1128/jcm.00659-11
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发表时间：
2011-07-01
影响因子：
9.4
通讯作者：
Garcia de Viedma, Dario
中科院分区：
文献类型：
--
作者：
Alonso, Maria;Jose Palacios, Juan;Garcia de Viedma, Dario
我们的研究就 rpoB 密码子 514 中的沉默取代的结核分枝杆菌利福平敏感菌株的传播发出了警报。在 1,450 个病例中，我们鉴定出了 12 个具有这种突变和相关限制性片段长度多态性 (RFLP) 类型的分离株。在三个独立的商业测定中，该突变损害了与野生型探针的杂交，这可能导致耐药性的错误分配。
Our study provides an alert regarding the transmission of rifampin-susceptible strains of Mycobacterium tuberculosis with a silent substitution in codon 514 of rpoB. Among 1,450 cases, we identified 12 isolates sharing this mutation and related restriction fragment length polymorphism (RFLP) types. The mutation impaired hybridization with the wild-type probes in three independent commercial assays, which could lead to misassignment of resistance.