Fully edited and partially edited nad9 transcripts differ in size and both are associated with polysomes in potato mitochondria.
Fully edited and partially edited nad9 transcripts differ in size and both are associated with polysomes in potato mitochondria.
复制标题
完全编辑和部分编辑的 nad9 转录本大小不同，并且都与马铃薯线粒体中的多核糖体相关。
DOI：
10.1093/nar/24.7.1369
复制
发表时间：
1996
影响因子：
14.9
通讯作者：
Hanson,MR
中科院分区：
文献类型：
--
作者：
Lu,B;Hanson,MR
Two classes ofnad9transcripts are present at different abundances in the steady-state RNA pool of potato mitochondria. The 5′- and 3′ termini of the transcripts were determined by primer extension and S1 nuclease protection analyses respectively. Using two primer pairs that will either specifically amplify the larger transcript or amplify both the larger and the smaller transcripts in RT-PCR analyses it was found that the largernad9transcripts are partially edited, while the smaller transcripts are fully edited. Both the larger and the smaller transcripts were found to be associated with mitochondrial polysomes. The polysome association was found to be sensitive to EDTA and puromycin treatment. Therefore, both fully and partially editednad9transcripts appear to be engaged in translation.
