Altered expression of interleukin 6 and interleukin 10 as a result of photodynamic therapy in vivo.
Altered expression of interleukin 6 and interleukin 10 as a result of photodynamic therapy in vivo.
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发表时间：
1997-09
期刊：
影响因子：
11.2
通讯作者：
S. Gollnick;Xiaonan Liu;B. Owczarczak;D. Musser;B. Henderson
中科院分区：
文献类型：
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作者：
S. Gollnick;Xiaonan Liu;B. Owczarczak;D. Musser;B. Henderson
光动力疗法（PDT）可以有效地破坏恶性组织，也诱导复杂的免疫应答，其增强抗肿瘤免疫，但也抑制皮肤接触超敏反应（CHS）和皮肤移植物存活。对这些作用的潜在机制知之甚少，但可能涉及细胞因子的介导。我们证明在BALB/c小鼠模型中，PDT递送到体内正常和肿瘤组织引起细胞因子白细胞介素（IL）-6和IL-10的表达的显着变化，但不是肿瘤坏死因子α。IL-6 mRNA和蛋白在PDT处理的EMT 6肿瘤中强烈增强。光动力疗法还增加了脾和皮肤中IL-6 mRNA的表达。这些数据表明，对PDT的一般炎症反应可能至少部分由IL-6介导。此外，IL-6可调节局部抗肿瘤免疫应答。相反，肿瘤中的IL-10 mRNA在PDT后降低。最重要的是，IL-10在暴露于强烈抑制CHS反应的PDT方案的小鼠的皮肤中被显著诱导，并且IL-10诱导的动力学与已知的CHS抑制动力学一致。我们认为，增强的IL-10表达在PDT后观察到的细胞介导的反应抑制中起作用。
Photodynamic therapy (PDT), which can effectively destroy malignant tissue, also induces a complex immune response that potentiates antitumor immunity but also inhibits skin contact hypersensitivity (CHS) and prolongs skin graft survival. The underlying mechanisms responsible for these effects are poorly understood but are likely to involve mediation by cytokines. We demonstrate in a BALB/c mouse model that PDT delivered to normal and tumor tissue in vivo causes marked changes in the expression of cytokines interleukin (IL)-6 and IL-10 but not tumor necrosis factor alpha. IL-6 mRNA and protein are strongly enhanced in the PDT-treated EMT6 tumor. PDT also increased IL-6 mRNA in exposed spleen and skin. These data suggest that the general inflammatory response to PDT may be mediated at least in part by IL-6. In addition, IL-6 may modulate the local antitumor immune response. In contrast, IL-10 mRNA in the tumor decreases following PDT. Most importantly, IL-10 is markedly induced in the skin of mice exposed to a PDT regime that strongly inhibits the CHS response, and the kinetics of IL-10 induction coincide with the known kinetics of CHS inhibition. We propose that the enhanced IL-10 expression plays a role in the observed suppression of cell-mediated responses seen following PDT.