Specificity of the mouse cytotoxic T lymphocyte response to adenovirus 5. E1A is immunodominant in H-2b, but not in H-2d or H-2k mice.
Specificity of the mouse cytotoxic T lymphocyte response to adenovirus 5. E1A is immunodominant in H-2b, but not in H-2d or H-2k mice.
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DOI：
10.4049/jimmunol.146.11.3977
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发表时间：
1991-06
影响因子：
4.4
通讯作者：
F. Rawle;B. Knowles;R. Ricciardi;V. Brahmacheri;P. Duerksen-Hughes;W. Wold;L. Gooding
中科院分区：
文献类型：
--
作者：
F. Rawle;B. Knowles;R. Ricciardi;V. Brahmacheri;P. Duerksen-Hughes;W. Wold;L. Gooding
本文研究了C57 BL/10（H-2b）、BALB/c（H-2d）和C3 H/HeJ（H-2k）三个品系小鼠对腺病毒5型（Ad 5）的抗原特异性和MHC限制性CTL反应。通过用活的Ad 5病毒体内致敏小鼠，然后用病毒感染的同基因细胞体外刺激脾细胞，制备多克隆Ad 5特异性CTL。Ad 5特异性CTL在C57 BL/10中为Db限制性CTL，在C3 H/HeJ中为Kk限制性CTL。在BALB/c小鼠中检测到Kd和Dd/Ld限制性CTL。在C57 BL/10小鼠中的多克隆Ad 5特异性CTL应答专门针对E1 A区域的产物，其仅占Ad 5基因组的5%。在BALB/c小鼠中，E1 A最多是一个非常小的靶标Ag，而在C3 H/HeJ小鼠中，E1 A根本不被识别。使用H-2同源小鼠品系B10.BR（H-2k）和C3H.SW（H-2b），表明E1 A的免疫优势是H-2依赖的。19 kDa的糖蛋白编码的E3区的Ad 5，结合到I类MHC的内质网，并防止其易位到细胞表面，不影响特异性的CTL反应在C57 BL/10小鼠对E1 A。然而，它影响了BALB/c小鼠中Ad 5特异性应答的MHC限制，选择性地抑制Kd限制性CTL的产生。
The Ag specificity and MHC restriction of the CTL response to adenovirus 5 (Ad5) in three strains of mice, C57BL/10 (H-2b), BALB/c (H-2d), and C3H/HeJ (H-2k), were tested. Polyclonal Ad5-specific CTL were prepared by priming mice in vivo with live Ad5 virus followed by secondary in vitro stimulation of the spleen cells with virus-infected syngeneic cells. The Ad5-specific CTL were Db restricted in C57BL/10 and Kk restricted in C3H/HeJ. In BALB/c mice both Kd- and Dd/Ld-restricted CTL were detected. The polyclonal Ad5-specific CTL response in C57BL/10 mice is directed exclusively against the products of the E1A region, which comprises only 5% of the Ad5 genome. In BALB/c mice E1A is at best a very minor target Ag and in C3H/HeJ mice E1A is not recognized at all. Using the H-2 congenic mouse strains B10.BR (H-2k) and C3H.SW (H-2b) it was shown that the immunodominance of E1A is H-2 dependent. The 19-kDa glycoprotein encoded in the E3 region of Ad5, which binds to class I MHC in the endoplasmic reticulum and prevents its translocation to the cell surface, does not affect the specificity of the CTL response in C57BL/10 mice toward E1A. However, it affects the MHC restriction of the Ad5-specific response in BALB/c mice, selectively inhibiting generation of Kd-restricted CTL.