Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33-97.
Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33-97.
复制标题
乳胶转录组的比较分析揭示了巴西橡胶树克隆 RRIM600 和 Reyan7-33−97 之间橡胶分子量差异的潜在机制
DOI：
10.1186/s12870-021-03022-5
复制
发表时间：
2021-05-29
影响因子：
5.3
通讯作者：
Huang T
中科院分区：
文献类型：
--
作者：
Xin S;Hua Y;Li J;Dai X;Yang X;Udayabhanu J;Huang H;Huang T
天然橡胶的加工性能和力学性能在很大程度上取决于其分子量（MW）和分子量分布（MWD）。然而，橡胶生物合成过程中分子量的调节机制仍不清楚。本研究以热研7 -33-97和RRIM 600橡胶树为试材，测定了1年生原始树和30年生定期割胶树胶乳的分子量和粒径。结果表明，两个无性系的胶乳分子量和粒径均随树龄的增加而增大。RRIM 600树胶乳的平均粒径小于同龄热研7 -33-97树胶乳的平均粒径。在1年生树木中，Reyan 7 -33-97乳胶的分子量略高于RRIM 600，而在30年生树木中，RRIM 600乳胶的分子量显着高于Reyan 7 -33-97乳胶。转录组图谱的比较分析表明，橡胶颗粒的平均大小与橡胶颗粒相关蛋白的表达水平呈负相关，胶乳的高分子量性状与异戊烯焦磷酸（IPP）单体生成途径基因和下游烯丙基二磷酸（APP）引发剂消耗非橡胶途径的增强表达密切相关。通过生物信息学分析，我们进一步鉴定了一组可能调控IPP生物合成的转录因子。总之，我们的研究结果揭示了潜在的调控机制，涉及IPP产生途径和非橡胶类异戊二烯途径的基因表达变化，影响IPP和APP引发剂的比例和含量，导致橡胶树无性系Reyan 7 -33-97和RRIM 600的同龄树之间的显着橡胶分子量的变化。本研究为进一步了解橡胶树的橡胶生物合成机制和橡胶品质的遗传改良奠定了基础。巴西的。在线版本包含补充材料，可通过10.1186/s12870-021-03022-5获得。
The processabilities and mechanical properties of natural rubber depend greatly on its molecular weight (MW) and molecular weight distribution (MWD). However, the mechanisms underlying the regulation of molecular weight during rubber biosynthesis remain unclear. In the present study, we determined the MW and particle size of latex from 1-year-old virgin trees and 30-year-old regularly tapped trees of the Hevea clones Reyan7-33–97 and RRIM600. The results showed that both the MW and the particle size of latex varied between these two clones and increased with tree age. Latex from RRIM600 trees had a smaller average particle size than that from Reyan7-33–97 trees of the same age. In 1-year-old trees, the Reyan7-33–97 latex displayed a slightly higher MW than that of RRIM600, whereas in 30-year-old trees, the RRIM600 latex had a significantly higher MW than the Reyan7-33–97 latex. Comparative analysis of the transcriptome profiles indicated that the average rubber particle size is negatively correlated with the expression levels of rubber particle associated proteins, and that the high-MW traits of latex are closely correlated with the enhanced expression of isopentenyl pyrophosphate (IPP) monomer-generating pathway genes and downstream allylic diphosphate (APP) initiator-consuming non-rubber pathways. By bioinformatics analysis, we further identified a group of transcription factors that potentially regulate the biosynthesis of IPP. Altogether, our results revealed the potential regulatory mechanisms involving gene expression variations in IPP-generating pathways and the non-rubber isoprenoid pathways, which affect the ratios and contents of IPP and APP initiators, resulting in significant rubber MW variations among same-aged trees of the Hevea clones Reyan7-33–97 and RRIM600. Our findings provide a better understanding of rubber biosynthesis and lay the foundation for genetic improvement of rubber quality in H. brasiliensis. The online version contains supplementary material available at 10.1186/s12870-021-03022-5.
登录
查看更多内容
DOI：
10.1093/dnares/dsw056
发表时间：
2017-04-01
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Makita Y;Ng KK;Veera Singham G;Kawashima M;Hirakawa H;Sato S;Othman AS;Matsui M
通讯作者：
Matsui M
对第一个塔拉萨克姆·萨格里兹转录组的分析揭示了潜在的橡胶产量相关的SNP。
影响因子：
4.6
作者：
Luo Z;Iaffaldano BJ;Zhuang X;Fresnedo-Ramírez J;Cornish K
通讯作者：
Cornish K
影响因子：
3.8
作者：
Collins-Silva, Jillian;Nural, Aise Taban;Shintani, David
通讯作者：
Shintani, David
影响因子：
6.9
作者：
Chow, Keng-See;Wan, Kiew-Lian;Yeang, Hoong-Yeet
通讯作者：
Yeang, Hoong-Yeet
影响因子：
5.3
作者：
Long, Xiangyu;Li, Heping;Tang, Chaorong
通讯作者：
Tang, Chaorong