Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison
Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison
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DOI：
10.1099/00207713-48-2-581
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发表时间：
1998-04-01
期刊：
影响因子：
--
通讯作者：
Brousseau, R
中科院分区：
文献类型：
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作者：
Chatellier, S;Harel, J;Brousseau, R
测定猪链球菌血清型1-34和1/2参考菌株的16S rRNA基因序列。比较序列分析表明，猪链球菌参考菌株序列相似度为93.94 ~ 100%。由相似矩阵构造树状图。代表32种血清型的32株毒株属于分为3个聚类的主要群体。而猪链球菌血清型32、33和34则距离较远。对更远的猪链球菌血清型20、22、26、32、33和34株的生化特征与其他猪链球菌血清型相似。猪链球菌参考菌株的16S rRNA基因序列与其他链球菌属成员的序列比较表明，除猪链球菌血清型32、33和34外，参考菌株未与该属其他种聚集。综上所述，16S rRNA基因序列分析确定了猪链球菌的主要参考菌株群，该菌株与猪链球菌的血清型32、33和34关系密切，且差异较大。然而，到目前为止，没有强有力的证据表明在其他物种中对这些血清型毒株进行重新分类。
The 16S rRNA gene sequences of reference strains of Streptococcus suis serotypes 1-34 and 1/2 were determined. A comparative sequence analysis showed that the degree of sequence similarity between S. suis reference strains ranged from 93.94 to 100%. A dendrogram was constructed from the similarity matrix. Thirty-two strains representing 32 serotypes fell into a major group divided into three clusters. The other strains, S.suis serotypes 32, 33 and 34, were more distant. Biochemical characterization of the six more distant strains, including S. suis serotypes 20, 22, 26, 32, 33 and 34, revealed a profile similar to that of other S. suis serotypes. Comparison of the 16S rRNA gene sequences of S. suis reference strains with sequences of other members of the genus Streptococcus indicated that with the exception of S. suis serotypes 32, 33 and 34, reference strains did not cluster with any other species in the genus. In conclusion, 16S rRNA gene sequence analysis defined a major group of S. suis reference strains which were very closely related and a higher divergence for S, suis serotypes 32, 33 and 34. However, to date, there is no strong evidence to reclassify strains of these serotypes in another species.