MAP6 is an intraluminal protein that induces neuronal microtubules to coil
MAP6 is an intraluminal protein that induces neuronal microtubules to coil
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DOI：
10.1126/sciadv.aaz4344
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发表时间：
2020-04-01
期刊：
影响因子：
13.6
通讯作者：
Delphin, Christian
中科院分区：
文献类型：
--
作者：
Cuveillier, Camille;Delaroche, Julie;Delphin, Christian
神经元的活动在很大程度上依赖于微管，微管塑造神经元过程并在其中运输无数分子。虽然通过生长和收缩事件不断重塑，但神经元微管必须足够稳定以维持神经系统布线。这种稳定性以某种方式由各种微管相关蛋白（MAP）维持，但对这些蛋白质如何工作知之甚少。在这里，我们表明，MAP6，以前已知赋予微管冷稳定性，促进生长。更出乎意料的是，MAP6定位在微管的内腔中，诱导微管卷曲成左旋螺旋，并在晶格中形成孔，可能释放机械应力。这些特征以前在微管中没有发现，并且可能在维持轴突宽度或在发育过程中面对压缩力时提供灵活性方面发挥作用。
Neuronal activities depend heavily on microtubules, which shape neuronal processes and transport myriad molecules within them. Although constantly remodeled through growth and shrinkage events, neuronal microtubules must be sufficiently stable to maintain nervous system wiring. This stability is somehow maintained by various microtubule-associated proteins (MAPs), but little is known about how these proteins work. Here, we show that MAP6, previously known to confer cold stability to microtubules, promotes growth. More unexpectedly, MAP6 localizes in the lumen of microtubules, induces the microtubules to coil into a left-handed helix, and forms apertures in the lattice, likely to relieve mechanical stress. These features have not been seen in microtubules before and could play roles in maintaining axonal width or providing flexibility in the face of compressive forces during development.