Mannosyltransferase is required for cell wall biosynthesis, morphology and control of asexual development in Neurospora crassa
Mannosyltransferase is required for cell wall biosynthesis, morphology and control of asexual development in Neurospora crassa
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DOI：
10.3852/mycologia.97.4.872
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发表时间：
2005-07-01
期刊：
影响因子：
2.8
通讯作者：
Free, SJ
中科院分区：
文献类型：
--
作者：
Bowman, SM;Piwowar, A;Free, SJ
两个脉孢菌突变体的表型，其中包括一个紧密的殖民地生长模式，不能形成分生孢子和不能形成原周壳已被分离和表征。相关的突变被定位到测序的脉孢菌基因组上的相同位点。然后，通过PCR扩增和测序测定在映射的基因座处检查来自突变体基因组的可能的候选基因来鉴定导致突变体表型的突变。结果表明，图谱和测序策略是一种可行的方法来鉴定新孢子菌中的突变基因。负责表型的基因是一个假定的α-1，2-甘露糖基转移酶基因。突变体细胞壁具有改变的组成，表明该基因在细胞壁生物合成中起作用。结果表明，mnt-1基因是正常细胞壁生物合成、形态和调节无性发育所必需的。
Two Neurospora mutants with a phenotype that includes a tight colonial growth pattern, an inability to form conidia and an inability to form protoperithecia have been isolated and characterized. The relevant mutations were mapped to the same locus on the sequenced Neurospora genome. The mutations responsible for the mutant phenotype then were identified by examining likely candidate genes from the mutant genomes at the mapped locus with PCR amplification and a sequencing assay. The results demonstrate that a map and sequence strategy is a feasible way to identify mutant genes in Neumspora. The gene responsible for the phenotype is a putative alpha-1,2-mannosyltransferase gene. The mutant cell wall has an altered composition demonstrating that the gene functions in cell wall biosynthesis. The results demonstrate that the mnt-1 gene is required for normal cell wall biosynthesis, morphology and for the regulation of asexual development.