Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.
Lactobacillus acidophilus and Bifidobacterium longum supernatants upregulate the serotonin transporter expression in intestinal epithelial cells.
复制标题
嗜酸乳杆菌和长双歧杆菌上清液上调肠上皮细胞中血清素转运蛋白的表达
DOI：
10.4103/sjg.sjg_333_17
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Wang YM
中科院分区：
文献类型：
--
作者：
Cao YN;Feng LJ;Wang BM;Jiang K;Li S;Xu X;Wang WQ;Zhao JW;Wang YM
背景/目的：益生菌具有缓解肠易激综合征(IBS)的作用；然而，其潜在机制尚不清楚。本研究旨在研究嗜酸乳杆菌和长双歧杆菌培养上清液对5-羟色胺转运体(SERT)信使核糖核酸(MRNA)和蛋白质表达的影响。材料和方法：分别用不同浓度的嗜酸乳杆菌和长链球菌培养上清液作用于HT-29和Caco-2细胞12h和24 h。用实时定量聚合酶链式反应(Real-time PCR)和Western-blotting检测SERT的mRNA和蛋白水平。结果：不同浓度的嗜酸乳杆菌和长链球菌培养上清液作用于HT-29和Caco-2细胞12 h和24 h后，其SERT基因的表达水平均高于对照组。此外，该蛋白在两种细胞中的表达也上调，与相应的mRNA的表达水平大致相同。结论：嗜酸乳杆菌和长杆菌培养上清液可上调肠上皮细胞SERT的mRNA和蛋白水平。
Background/Aims: Probiotics play a role in relieving irritable bowel syndrome (IBS); however, the underlying mechanism is yet unclear. The aim of the study was to investigate the effects of the supernatants of Lactobacillus acidophilus and Bifidobacterium longum on the expression of serotonin transporter (SERT) messenger ribonucleic acid (mRNA) and protein. Materials and Methods: HT-29 and Caco-2 cells were treated with different concentrations of L. acidophilus and B. longum supernatants for 12 h and 24 h, respectively. SERT mRNA and proteins levels were detected by real-time polymerase chain reaction (real-time PCR) and Western-blotting. Results: The mRNA levels of SERT in HT-29 and Caco-2 cells treated with different concentrations of L. acidophilus or B. longum supernatants for 12 h and 24 h, each, were higher than that in the control groups. In addition, the expression of the protein in both cells was also upregulated, which was approximately similar to that of the corresponding mRNA. Conclusions: L. acidophilus and B. longum supernatants can upregulate SERT mRNA and protein levels in intestinal epithelial cells.