Telocytes in Pregnancy-Induced Physiological Liver Growth
Telocytes in Pregnancy-Induced Physiological Liver Growth
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妊娠诱导的生理性肝脏生长中的特细胞
DOI：
10.1159/000374068
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Yang, Changqing
中科院分区：
文献类型：
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作者：
Wang, Fei;Bei, Yihua;Yang, Changqing
背景/目标：我们先前记录了肝中端细胞（TC）的存在，并进一步表明TC在肝切除术后肝再生中的潜在作用。妊娠诱导的肝脏生长，而不是肝切除术后的肝脏再生，是一种生理性的肝脏适应，以满足增强的营养和代谢需求。然而，TC在妊娠诱导的肝脏生长中的可能作用仍然未知。方法：在不同时间点（妊娠第0.5、4.5、8.5、10.5、12.5、14.5、16.5和18.5天）处死妊娠小鼠。以肝脏重量评价妊娠期肝脏生长情况。采用白蛋白和5-乙炔基-2 '-脱氧尿苷（EdU）免疫双标法检测肝细胞增殖，采用CD 34/PDGFR-α免疫双标法计数TC。结果：妊娠诱导的肝脏生长之前，在妊娠第4.5、8.5、14.5和16.5天肝细胞增殖增加。CD 34/PDGFR-α免疫双标记法检测到妊娠4.5 d和14.5 d小鼠肝脏中TC数量显著增加，与妊娠期间肝细胞增殖出现两个高峰相一致。结论：我们的研究结果表明，TC和肝细胞增殖在妊娠诱导的肝脏生长的可能关系。
Background/Aims: We previously documented the presence of Telocytes (TCs) in liver and further indicated the potential roles of TCs in liver regeneration after hepatectomy. Pregnancy-induced liver growth, other than liver regeneration after hepatectomy, is a physiological hepatic adaption to meet the enhanced nutritional and metabolic demands. However, the possible roles of TCs in pregnancy-induced liver growth remain unknown. Methods: Pregnant mice were sacrificed at different time points (pregnancy day 0.5, 4.5, 8.5, 10.5, 12.5, 14.5, 16.5, and 18.5). The liver weight was used to evaluate the liver growth during pregnancy. Hepatocytes proliferation was determined by albumin and 5-ethynyl-2'- deoxyuridine (EdU) double immunostaining while TCs were counted by double immunolabeling for CD34/PDGFR-α. Results: Pregnancy-induced liver growth was preceded by increased proliferation of hepatocytes at pregnancy day 4.5, 8.5, 14.5 and 16.5. Furthermore, the number of TCs in liver detected by double immunolabeling for CD34/PDGFR-α was significantly increased at pregnancy day 4.5 and day 14.5, that was coincident with the occurrence of two peaks of hepatic cell proliferation during pregnancy. Conclusion: Our results suggest a possible relationship between TCs and hepatocyte proliferation in pregnancy-induced liver growth.