Nucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix
Nucleolar segregation during apoptosis of haemopoietic stem cell line FDCP-Mix
复制标题
DOI：
10.1038/sj.cdd.4400500
复制
发表时间：
1999-04-01
影响因子：
12.4
通讯作者：
Allen, TD
中科院分区：
文献类型：
--
作者：
Reipert, S;Bennion, G;Allen, TD
细胞凋亡过程中细胞的程序性消除在形态学和生物化学上都不同于坏死。目前，细胞凋亡的形态学描述区分核仁的分离和所谓的“染色质浓缩”，后者源于对凋亡细胞核的核膜附近的电子致密物质的观察，虽然有足够的证据表明DNA参与了电子致密边缘，但它们的真实性质仍然未知。通过研究多能小鼠造血干细胞系FDCP-Mix的凋亡，我们发现形态学和组织化学证据表明，在核膜（NE）的电子致密物质已经出现作为与核膜相关的核仁分离的结果。剩余的电子致密和均匀的核仁标记RNA酶-金，但更强烈的DNA酶-金，因此可能被误认为是“浓缩的染色质”在光学显微镜下。FDCP-Mix中DNA酶金的电子致密材料的标记可以通过在细胞死亡的早期阶段DNA迁移到大量核仁中来解释。
The programmed elimination of cells during apoptosis is distinct from necrosis both morphologically and biochemically, Currently, the morphological description of apoptosis discriminates between the segregation of the nucleolus and the so called 'chromatin condensation', The latter originates from observations of electron dense material adjacent to the nuclear envelope of apoptotic nuclei, Although there is ample evidence for an involvement of DNA in electron dense marginations, their true nature is still unknown. By studying apoptosis in FDCP-Mix, a pluripotent murine haemopoietic stem cell line, we found morphological and histochemical evidence that electron dense material at the nuclear envelope (NE) has emerged as a result of the segregation of nucleoli in association with the nuclear membrane. The remaining electron dense and homogenous bulk of the nucleolus labels for RNAse-gold, but even more intensely for DNAse-gold, and therefore could possibly be mistaken as 'condensed chromatin' in the light microscope. The labelling of the electron dense material for DNase gold in FDCP-Mix could be explained by a migration of DNA into the bulk of the nucleoli at an early stage of cell death.