Effects of Cu/Zn superoxide dismutase on strain injury-induced oxidative damage to skeletal muscle in rats
Effects of Cu/Zn superoxide dismutase on strain injury-induced oxidative damage to skeletal muscle in rats
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DOI：
10.33549/physiolres.930614
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发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Wang, S
中科院分区：
文献类型：
--
作者：
Li, GZ;Feng, X;Wang, S
本研究旨在探讨补充超氧化物歧化酶（SOD）是否能减轻应变性骨骼肌氧化损伤。实验动物右侧腓肠肌拉伤模型。SOD治疗组自伤后开始每天灌胃Cu/ZnSOD 10 000 U/kg体重，对照组灌胃生理盐水。伤后3、7天检测血浆抗氧化指标和肌肉损伤指标。将损伤肌肉取出固定，进行组织学观察和结蛋白免疫组化检测。结果表明，SOD组大鼠血浆SOD、谷胱甘肽过氧化物酶（GSH-Px）、总抗氧化能力（T-AOC）水平在第3、7天均显著高于生理盐水组，而血浆肌酸激酶（CK）、丙二醛（MDA）水平则显著低于生理盐水组。肌肉切片的组织学检查显示，SOD组的损伤程度较低，其中结蛋白的表达水平高于生理盐水组。提示补充SOD可减轻牵张性肌肉损伤，促进其再生。
This study was designed to determine whether the supplement of superoxide dismutase (SOD) could attenuate strain-induced oxidative damage to skeletal muscle in rats. Experimental animals were injured in right gastrocnemius muscles by a strain injury model. SOD-treated groups were given Cu/Zn SOD 10 000 U/kg body weight per day since injured, while control groups were given normal saline. Parameters of antioxidant and muscle damage were detected in plasma 3 and 7 days postinjury. The injured muscles were removed and fixed for histology observation and immunohistochemistry assay of desmin. The results showed that plasma levels of SOD, glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) in SOD group were significantly higher than in the saline group on day 3 or 7, while the plasma creatine kinase (CK) and malondialdehyde (MDA) were lower in the SOD group than in the saline group. The histological examination of muscle sections revealed a lower degree of damage in the SOD group in which the expression level of desmin was higher than in the saline group. It is suggested that SOD supplement may attenuate strain-induced muscle damage and facilitate its regeneration.