A Novel Stress-Induced Sugarcane Gene Confers Tolerance to Drought, Salt and Oxidative Stress in Transgenic Tobacco Plants
A Novel Stress-Induced Sugarcane Gene Confers Tolerance to Drought, Salt and Oxidative Stress in Transgenic Tobacco Plants
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DOI：
10.1371/journal.pone.0044697
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发表时间：
2012-09-11
期刊：
影响因子：
3.7
通讯作者：
Menossi, Marcelo
中科院分区：
文献类型：
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作者：
Begcy, Kevin;Mariano, Eduardo D.;Menossi, Marcelo
背景：干旱是影响全球作物生产力的主要非生物胁迫。甘蔗可以承受在茎成熟的最后阶段期间的缺水时期，在此期间发生蔗糖积累。同时，长时间的干旱可能会导致严重的plant loss.Methodology/Principal Findings：在以前的研究中，我们评估了干旱胁迫植物的转录组，以更好地了解甘蔗对干旱的反应。在上调的基因中有Scdr 1（甘蔗干旱响应1）。这里报道的研究的目的是描述这个基因。scdr 1编码一个含有248个氨基酸的蛋白质，含有大量的脯氨酸（19%）和半胱氨酸（13%）残基。系统发育分析表明，ScDR 1与其他单子叶植物的同源物同属一个分支，与双子叶植物的同源物不同。Scdr 1基因在不同品种甘蔗中的表达与耐旱性没有明显的相关性。结论：Scdr 1基因在转基因烟草中的过表达增强了其对干旱、盐胁迫和氧化胁迫的耐受性，表现为光合作用、含水量、生物量、发芽率、叶绿素含量的增加和活性氧积累的减少。与野生型植株相比，转Scdr 1基因植株的蒸腾速率（E）、净光合速率（A）、气孔导度（gs）和内部叶片CO2浓度等生理指标受非生物胁迫的影响较小。总的来说，我们的研究结果表明，Scdr 1赋予多种非生物胁迫的耐受性，突出了该基因的生物技术应用的潜力。
Background: Drought is a major abiotic stress that affects crop productivity worldwide. Sugarcane can withstand periods of water scarcity during the final stage of culm maturation, during which sucrose accumulation occurs. Meanwhile, prolonged periods of drought can cause severe plant losses.Methodology/Principal Findings: In a previous study, we evaluated the transcriptome of drought-stressed plants to better understand sugarcane responses to drought. Among the up-regulated genes was Scdr1 (sugarcane drought-responsive 1). The aim of the research reported here was to characterize this gene. Scdr1 encodes a putative protein containing 248 amino acids with a large number of proline (19%) and cysteine (13%) residues. Phylogenetic analysis showed that ScDR1is in a clade with homologs from other monocotyledonous plants, separate from those of dicotyledonous plants. The expression of Scdr1 in different varieties of sugarcane plants has not shown a clear association with drought tolerance.Conclusions/Significance: The overexpression of Scdr1 in transgenic tobacco plants increased their tolerance to drought, salinity and oxidative stress, as demonstrated by increased photosynthesis, water content, biomass, germination rate, chlorophyll content and reduced accumulation of ROS. Physiological parameters, such as transpiration rate (E), net photosynthesis (A), stomatal conductance (gs) and internal leaf CO2 concentration, were less affected by abiotic stresses in transgenic Scdr1 plants compared with wild-type plants. Overall, our results indicated that Scdr1 conferred tolerance to multiple abiotic stresses, highlighting the potential of this gene for biotechnological applications.