Gonadotropin and cAMP modulation of IGE binding protein production in ovarian granulosa cells.
Gonadotropin and cAMP modulation of IGE binding protein production in ovarian granulosa cells.
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DOI：
10.1152/ajpendo.1992.262.4.e497
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发表时间：
1992-04
期刊：
影响因子：
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通讯作者：
R. Grimes;S. Samaras;J. Barber;S. Shimasaki;N. Ling;J. Hammond
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文献类型：
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作者：
R. Grimes;S. Samaras;J. Barber;S. Shimasaki;N. Ling;J. Hammond
猪颗粒细胞（GC）在培养中产生胰岛素样生长因子（IGF）结合蛋白（BP）-3和IGFBP-2。促性腺激素，促卵泡激素（FSH），显着抑制GC生产这些IGFBPs在控制文化和文化刺激胰岛素加表皮生长因子（EGF）或IGF-I加EGF。腺苷酸环化酶刺激剂（毛喉素，霍乱毒素）和腺苷3 '，5'-环一磷酸（cAMP）的衍生物，8-溴腺苷3 '，5'-环一磷酸，以类似于FSH的方式抑制IGFBP的合成。相反，与对照相比，cAMP作用的拮抗剂（R）-p-腺苷3 '，5'-环硫代磷酸酯[（R）-p-cAMPS]显著刺激IGFBP-3和IGFBP-2的产生。（R）-p-cAMPS的这种刺激作用被FSH以剂量依赖性方式共同处理所抵消。最后，用FSH加3-异丁基-1-甲基黄嘌呤处理GC培养物导致IGFBP-3 mRNA编码的细胞含量显著减少，而IGFBP-2 mRNA没有变化。总之，发现升高细胞内cAMP的药物模拟FSH对IGFBP产生的作用。
Porcine granulosa cells (GC) produce insulin-like growth factor (IGF) binding protein (BP)-3 and IGFBP-2 in culture. A gonadotropin, follicle-stimulating hormone (FSH), dramatically inhibited GC production of these IGFBPs in control cultures and in cultures stimulated by insulin plus epidermal growth factor (EGF) or IGF-I plus EGF. Stimulators of adenylate cyclase (forskolin, cholera toxin) and a derivative of adenosine 3',5'-cyclic monophosphate (cAMP), 8-bromoadenosine 3',5'-cyclic monophosphate, inhibited IGFBP synthesis in a manner similar to FSH. In contrast, the antagonist of cAMP action, (R)-p-adenosine 3',5'-cyclic phosphorothioate [(R)-p-cAMPS], significantly stimulated production of IGFBP-3 and IGFBP-2 compared with controls. This stimulatory effect of (R)-p-cAMPS was counteracted by cotreatment with FSH in a dose-dependent manner. Finally, treatment of GC cultures with FSH plus 3-isobutyl-1-methylxanthine resulted in a significant reduction in cellular content of mRNA coding for IGFBP-3 with no change in IGFBP-2 mRNA. In summary, agents that elevate intracellular cAMP were found to mimic the effects of FSH on IGFBP production.