Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits
Respiratory and C4-photosynthetic NAD-malic enzyme coexist in bundle sheath cells mitochondria and evolved via association of differentially adapted subunits
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呼吸酶和 C4 光合 NAD-苹果酸酶共存于束鞘细胞线粒体中，并通过差异适应亚基的关联而进化
DOI：
10.1101/2021.06.16.448762
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Maurino VG
中科院分区：
文献类型：
--
作者：
Hüdig M;Tronconi MA;Zubimendi JP;Sage TL;Poschmann G;Bickel D;Gohlke H;Maurino VG
在植物线粒体中，烟酰胺腺嘌呤二核苷酸-苹果酸酶（NAD-ME）在苹果酸呼吸中具有管家功能。在不同的植物谱系中，NAD-ME在C4光合作用中是独立的。在C4醉蝶花属植物Gynandropsis gynandra和狭叶醉蝶花中，所有NAD-ME基因（NAD-MEα、NAD-MEβ1和NAD-ME β2）均受到C4进化的影响，并且表达水平高于其在C3植物Tarenaya hassleriana中的直向同源物。在哈氏嗜热菌中，NAD-ME管家功能由两种异聚体NAD-MEα/β1和NAD-MEα/β2执行，具有相似的生化特性。在两种C4物质中，该作用仅限于NAD-MEα/β2。在C4物种中，NAD-MEα/β1只存在于叶片中，它是C4脱羧酶的主要活性来源，GgNAD-ME α/β1（GgNAD-MEα/β1）具有很高的催化活性，并能被C4中间体天冬氨酸差异激活，证实它是C4脱羧酶。在C4进化过程中，NAD-MEβ1失去了催化活性，其对酶活性的贡献来自于对相关α亚基的稳定作用和获得的调节特性。我们的结论是，在束鞘细胞线粒体的C4物种，NAD-ME作为C4光合脱羧酶和管家酶的功能共存，并通过联合收割机结合相同的α-亚基与差异适应的β-亚基的异构体执行。
In plant mitochondria, nicotinamide adenine dinucleotide-malic enzyme (NAD-ME) has a housekeeping function in malate respiration. In different plant lineages, NAD-ME was independently co-opted in C4photosynthesis. In the C4Cleome species,Gynandropsis gynandraandCleome angustifolia, all NAD-ME genes (NAD-MEα,NAD-MEβ1, andNAD-MEβ2) were affected by C4evolution and are expressed at higher levels than their orthologs in the C3speciesTarenaya hassleriana. InT. hassleriana, the NAD-ME housekeeping function is performed by two heteromers, NAD-MEα/β1 and NAD-MEα/β2, with similar biochemical properties. In both C4species, this role is restricted to NAD-MEα/β2. In the C4species, NAD-MEα/β1 is exclusively present in the leaves, where it accounts for most of the enzymatic activity.Gynandropsis gynandraNAD-MEα/β1 (GgNAD-MEα/β1) exhibits high catalytic efficiency and is differentially activated by the C4intermediate aspartate, confirming its role as the C4-decarboxylase. During C4evolution, NAD-MEβ1 lost its catalytic activity; its contribution to the enzymatic activity results from a stabilizing effect on the associated α-subunit and the acquisition of regulatory properties. We conclude that in bundle sheath cell mitochondria of C4species, the functions of NAD-ME as C4photosynthetic decarboxylase and as a housekeeping enzyme coexist and are performed by isoforms that combine the same α-subunit with differentially adapted β-subunits.
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影响因子：
7.2
作者：
Ermakova, Maria;Danila, Florence R.;von Caemmerer, Susanne
通讯作者：
von Caemmerer, Susanne
影响因子：
5.6
作者：
Tronconi MA;Hüdig M;Schranz ME;Maurino VG
通讯作者：
Maurino VG
影响因子：
5.5
作者：
Maier JA;Martinez C;Kasavajhala K;Wickstrom L;Hauser KE;Simmerling C
通讯作者：
Simmerling C
DOI：
--
发表时间：
2005
期刊：
影响因子：
--
作者：
Miyawaki A;Nagai T;Mizuno H.
通讯作者：
Mizuno H.
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影响因子：
2.9
作者：
Dali Liu;C. Hwang;P. Cook
通讯作者：
P. Cook