ELECTRICAL STIMULATIONS OF PERIFUSED MAGNOCELLULAR NUCLEI INVITRO ELICIT CA-2+-DEPENDENT, TETRODOTOXIN-INSENSITIVE RELEASE OF OXYTOCIN AND VASOPRESSIN
ELECTRICAL STIMULATIONS OF PERIFUSED MAGNOCELLULAR NUCLEI INVITRO ELICIT CA-2+-DEPENDENT, TETRODOTOXIN-INSENSITIVE RELEASE OF OXYTOCIN AND VASOPRESSIN
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DOI：
10.1016/0304-3940(87)90717-8
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发表时间：
1987-05-06
影响因子：
2.5
通讯作者：
RICHARD, P
中科院分区：
文献类型：
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作者：
DISCALAGUENOT, D;STROSSER, MT;RICHARD, P
体外灌流大鼠室旁核(PVN)和视上核(SON)，用放射免疫法测定静息和电刺激时催产素和加压素的释放。刺激频率为10 Hz(10次S脉冲，每10次S刺激5min)、强度为4 mA时，只有长脉冲(10ms)才能引起显著的激素释放。在不同频率(10、20、40或80赫兹)和不同强度(4、5、10或20 mA)下施加的短脉冲(1ms)不起作用。钠通道阻滞剂河豚毒素(TTX)不影响这两种激素的电刺激释放，但在低钙介质或钙通道阻滞剂盐酸加洛帕米(D-600)的存在下可被阻断。这些结果表明，在电刺激后，即使在阻断动作电位的情况下，催产素和加压素也会在大细胞内局部释放。讨论了树枝晶释放的可能性。
Isolated rat paraventricular (PVN) and supraoptic (SON) nuclei were perifused in vitro and oxytocin and vasopressin releases were measured by radioimmunoassay during rest and during electrical stimulation. Stimulations at a frequency of 10 Hz (10-s bursts, every 10 s for 5 min) and an intensity of 4 mA, induced significant hormone release only with long duration pulses (10 ms). Short pulses (1 ms) applied at various frequencies (10, 20, 40 or 80 Hz) and intensities (4, 5, 10 or 20 mA) had no effect. The electrically evoked release of both hormones was not affected by tetrodotoxin (TTX), a sodium channel blocker, but was blocked in low-calcium medium or in the presence of gallopamil hydrochloride (D-600), a calcium channel blocker. These results suggest that, following electrical stimulation, oxytocin and vasopressin are released locally within the magnocellular nuclei even when blocking action potentials. The possibility of dendritic release is discussed.