Age-related NADPH Oxidase (arNOX) Activity Correlated with Cartilage Degradation and Bony Changes in Age-related Osteoarthritis.
Age-related NADPH Oxidase (arNOX) Activity Correlated with Cartilage Degradation and Bony Changes in Age-related Osteoarthritis.
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DOI：
10.3346/jkms.2015.30.9.1246
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发表时间：
2015-09
影响因子：
4.5
通讯作者：
Song IH
中科院分区：
文献类型：
--
作者：
Kim MJ;Kim HJ;Hong YH;Lee CK;Kim YW;Shon OJ;Song IH
本研究旨在探讨老年性膝骨性关节炎(OA)患者的年龄相关NADPH氧化酶(ArNOX)活性。用氧化铁细胞色素C还原测定法测定血清和软骨中arNOX活性。通过全膝关节置换术获得的全层膝关节软骨按OB分级。膝关节X线片按Kellgren-Lawrence(K/L)分级标准判定膝关节骨关节炎的严重程度。软骨β-半乳糖苷酶、低氧诱导因子-1α和GLUT-1表达水平被评估为组织衰老、缺氧和糖酵解的标志。软骨β-半乳糖苷酶、HIF-1α和GLUT-1水平越高，arNOX活性越高(P=0.002)。软骨表面有缺损者(OBⅡ、III级)的arNOX活性高于无表面缺损者(OB 0、I级)(P=0.012)。软骨arNOx活性与血清arNOx活性呈正相关(r=-0.577，P=0.023)。双侧ROA组血清arNOX活性显著高于无ROA组和单侧ROA组(分别为2.449±0.81、2.022±0.251 nM/m L，P=0.019)。本研究结果表明，骨关节炎本身并不是导致arNOX活性升高的原因，然而，arNOX过度活动与软骨高度退化以及年龄相关性骨关节炎的高程度和高程度的ROA有关。
The purpose of this study was to investigate the age-related NADPH oxidase (arNOX) activity in patients with age-related knee osteoarthritis (OA). Serum and cartilage arNOX activities were determined using an oxidized ferricytochrome C reduction assay. Full-thickness knee joint cartilages obtained through total knee replacement surgery were graded according to the Outerbridge (OB) classification. Radiographic severity of OA was determined on Knee X-rays according to the Kellgren-Lawrence (K/L) grading system. Cartilage β-galactosidase, HIF-1α, and GLUT-1 expression levels were evaluated as markers for tissue senescence, hypoxia, and glycolysis. Higher arNOX activities occurred with higher levels of cartilage β-galactosidase, HIF-1α, and GLUT-1 (P = 0.002). arNOX activity in cartilages with surface defects (OB grade II, III) was higher than in those without the defects (OB grade 0, I) (P = 0.012). Cartilage arNOX activity showed a positive correlation with serum arNOX activity (r = -0.577, P = 0.023). Serum arNOX activity was significantly higher in the OA subgroup with bilateral ROA than in the OA with no or unilateral ROA (2.449 ± 0.81, 2.022 ± 0.251 nM/mL, respectively, P = 0.019). The results of this study demonstrate that OA itself is not a cause to increase arNOX activities, however, arNOX hyperactivity is related to a high degree of cartilage degradation, and a high grade and extent of ROA in age-related OA.