Clinical significance of the low expression of FER1L4 in gastric cancer patients
Clinical significance of the low expression of FER1L4 in gastric cancer patients
复制标题
胃癌患者FER1L4低表达的临床意义
DOI：
10.1007/s13277-014-2259-4
复制
发表时间：
2014-10-01
期刊：
影响因子：
--
通讯作者：
Guo, Junming
中科院分区：
文献类型：
--
作者：
Liu, Zhong;Shao, Yongfu;Guo, Junming
长链非编码RNA（longnon-codingRNA，lncRNA）是一类长度大于200个核苷酸的新型调控性非编码RNA。近年来的研究发现，调控异常的lncRNA与人类肿瘤的发生有着密切的关系。然而，其临床意义在很大程度上是未知的。在这项研究中，我们研究了lncRNA-Fer-1-like蛋白4（FER 1 L4）在胃癌组织和血浆中的水平。通过实时定量逆转录酶-聚合酶链反应（qRT-PCR）测定人体组织和血浆中的FER 1 L4水平。然后，评估组织或血浆FER 1 L4水平与临床病理因素之间的相关性。构建受试者工作特征（ROC）曲线用于区分GC患者和对照组。与癌旁正常组织相比，91.80%（56/61）的胃癌组织中FER 1 L4表达水平显著降低。FER 1 L4低水平与肿瘤大小有关（p< 0.001），组织学分级（p= 0.001），一般分类（p< 0.001），浸润深度（p< 0.001），淋巴结转移（p< 0.001）、远处转移（p= 0.003）、TNM分期（p< 0.001）、血管或神经侵犯（p< 0.001或p = 0.003）和血清CA 72 -4（p< 0.001）。ROC曲线下面积（AUC）达0.778（p< 0.001），敏感性和特异性分别为67.2%和80.3%。此外，通过qRT-PCR检测血浆FER 1 L4。我们的数据显示，健康人和术前胃癌患者之间的血浆FER 1 L4水平没有差异，63.9%（53/83）的胃癌患者在手术后2周急剧下降（p= 0.028）。FER 1 L4可能在胃癌发生发展中起重要作用，并可能成为一种新的临床预后评估的生物标志物。
Long non-coding RNA (lncRNA) is a new class of regulative non-coding RNA, with a length larger than 200 nucleotides. Recent studies found that there are close relations between disregulative lncRNAs and human tumors. However, the clinical significances are largely unknown. In this study, we investigated the lncRNA–Fer-1-like protein 4 (FER1L4) level in gastric cancer tissues and plasma. The FER1L4 level in human tissues and plasma were measured by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Then, the correlations between the tissue or plasma FER1L4 levels and clinicopathological factors were assessed. A receiver operating characteristic (ROC) curve was constructed for differentiating GC patients from controls. Compared to matched adjacent non-tumorous tissues, FER1L4 expression levels in 91.80 % (56/61) of gastric cancer tissues are significantly decreased. The low FER1L4 level were associated with tumor size (p< 0.001), histologic grade (p= 0.001), general classification (p< 0.001), depth of invasion (p< 0.001), lymphatic metastasis (p< 0.001), distant metastasis (p= 0.003), TNM stage (p< 0.001), vessel or nerve invasion (p< 0.001 orp= 0.003), and serum CA72-4 (p< 0.001). The area under the ROC curve (AUC) was up to 0.778 (p< 0.001) and the sensitivity and specificity were 67.2 and 80.3 %, respectively. Also, plasma FER1L4 was detected by qRT-PCR. Our data show that there was no difference of plasma FER1L4 level between healthy person and preoperative gastric cancer patients, with a sharp decline in 63.9 % (53/83) of gastric cancer patients 2 weeks after surgery (p= 0.028). Taken together, FER1L4 might play a crucial role in human gastric cancer and may be a new potential biomarker for clinical prognosis evaluation.