Mitochondrial DNA sequence polymorphisms of five ethnic populations from northern China
Mitochondrial DNA sequence polymorphisms of five ethnic populations from northern China
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DOI：
10.1007/s00439-003-1004-7
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发表时间：
2003-10-01
期刊：
影响因子：
5.3
通讯作者：
Zhang, YP
中科院分区：
文献类型：
--
作者：
Kong, QP;Yao, YG;Zhang, YP
为了研究中国北部5个民族(达吾尔族45人，鄂温克族47人，朝鲜族48人，蒙古族48人，鄂伦春族44人)线粒体DNA的多态性，我们采用直接测序或限制性片段长度多态性分析的方法，分析了控制区序列，并对编码区(特别是14576-16047区)的一些特征突变进行了分型。除了14个个体属于欧洲特有的单倍群R2、H、J和T外，所考虑的mtDNA可以归入最近描述的东亚特有的单倍群。除了我们先前研究中报道的10171-10659和14055-14590区域外，细胞色素b序列的多态被发现对定义或支持东亚mtDNA的单倍群状态非常有用。5个民族群体的单倍群分布频率不同，但总体上都含有大量的北方流行单倍群，如D、G、C和Z，这与其北方起源的民族史是一致的。人口普查较少的鄂温克和鄂伦春两个种群的母系遗传结构也表现出一致的特征，遗传多样性较低。
To study the mitochondrial DNA (mtDNA) polymorphisms in a total of 232 individuals from five ethnic populations (Daur, n=45; Ewenki, n=47; Korean, n=48; Mongolian, n=48; Oroqen, n=44) in northern China, we analyzed the control region sequences and typed for a number of characteristic mutations in coding regions (especially the region 14576-16047), by direct sequencing or restriction-fragment-length-polymorphism (RFLP) analysis. With the exception of 14 individuals belonging to the European-specific haplogroups R2, H, J, and T, the mtDNAs considered could be assigned into the East Asian-specific haplogroups described recently. The polymorphisms in cytochrome b sequence were found to be very informative for defining or supporting the haplogroups status of East Asian mtDNAs in addition to the reported regions 10171-10659 and 14055-14590 in our previous study. The haplogroup distribution frequencies varied in the five ethnic populations, but in general they all harbored a large amount of north-prevalent haplogroups, such as D, G, C, and Z, and thus were in agreement with their ethnohistory of northern origin. The two populations (Ewenki and Oroqen) with small population census also show concordant features in their matrilineal genetic structures, with lower genetic diversities observed.