Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater
Chlorella sorokiniana UTEX 2805, a heat and intense, sunlight-tolerant microalga with potential for removing ammonium from wastewater
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DOI：
10.1016/j.biortech.2007.09.065
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发表时间：
2008-07-01
影响因子：
11.4
通讯作者：
Bashan, Yoav
中科院分区：
文献类型：
--
作者：
de-Bashan, Luz E.;Trejo, Adan;Bashan, Yoav
2003 年夏天，在墨西哥不列颠哥伦比亚省拉巴斯处理设施的废水稳定池中，从大量绿色微藻华中分离出了一种微藻菌株。主要环境条件为气温超过40℃，水温37℃，中午水面日照量高达2400μmolm(2)s(-1)，持续数小时，持续四个月。该微藻被鉴定为Chlorella sorokiniana Shih。 Krauss 等人基于对其整个 18S rRNA 基因进行测序。在受控光生物反应器中，该菌株可以在40-42℃的温度和2500μmol·m(2)·s(-1)的光照强度下每天2s(-1)内在合成废水中生长到高种群密度，持续5h，并在这些条件下比正常较低温度(28℃)和较低的光照强度(60μmol·m(2)·s(-1))更好地有效去除废水中的铵。当与促进微藻生长的巴西固氮螺菌共固定时，微藻种群生长得更快，并去除了更多的铵。在暴露于极端生长条件下，共固定培养物中四种光合色素的数量增加。这种微藻菌株在极端温度和光照强度条件下具有作为废水处理剂的潜力。 (C) 2007 Elsevier Ltd. 保留所有权利。
In the summer of 2003, a microalga strain was isolated from a massive green microalgae bloom in wastewater stabilization ponds at the treatment facility of La Paz, B.C.S., Mexico. Prevailing environmental conditions were air temperatures over 40 degrees C, water temperature of 37 degrees C, and insolation of up to 2400 mu mol m(2) s(-1) at midday for several hours at the water surface for four months. The microalga was identified as Chlorella sorokiniana Shih. et Krauss, based on sequencing its entire 18S rRNA gene. In a controlled photo-bioreactor, this strain can grow to high population densities in synthetic wastewater at temperatures of 40-42 degrees C and light intensity of 2500 mu mol in 2 s(-1) for 5 h daily and efficiently remove ammonium from the wastewater under these conditions better than under normal lower temperature (28 degrees C) and lower light intensity (60 mu mol m(2) s(-1)). When co-immobilized with the bacterium Azospirillum brasilense that promotes growth of microalgae, the population of microalga grew faster and removed even more ammonium. Under exposure to extreme growth conditions, the quantity of four photosynthetic pigments increased in the co-immobilized cultures. This strain of microalga has potential as a wastewater treatment agent under extreme conditions of temperature and light intensity. (C) 2007 Elsevier Ltd. All rights reserved.