Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur)
Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur)
复制标题
棉蝽 Apolygus lucorum (Meyer-Dur) 蜕皮激素受体亚型 A 的分子和功能表征
DOI：
10.1016/j.gene.2015.07.085
复制
发表时间：
2015-12-10
期刊：
影响因子：
3.5
通讯作者：
Bai, Li-Xin
中科院分区：
文献类型：
--
作者：
Tan, Yong-An;Xiao, Liu-Bin;Bai, Li-Xin
我们从盲蝽（Apolygus lucorum, AlEcR-A）中克隆了蜕皮激素受体（EcR）亚型a的cDNA。AlEcR-A cDNA具有1410 bp的开放阅读框，在其a / b特异结构域的羧基端有大约20个氨基酸的保守序列。系统发育分析表明，AlEcR-A基因与其他半翅目物种的EcR-A基因非常相似。AlEcR-A mRNA在绿盲蝽发育的各个阶段均有表达，其峰值与表皮甾体激素脉冲相关。AlEcR-A在所有组织中均有表达，在表皮和脂肪体中表达量最大。northern blot分析在所有组织中检测到1.8 kb大小的AlEcR-A mRNA。我们利用RNAi技术在体内研究了AlEcR-A在黄菖蒲生长发育中的功能。AlEcR-A特异性反义RNA处理显著降低了5龄若虫的体重。三龄若虫到成虫的死亡率随着龄期的显著增加而显著增加。(C) 2015 Elsevier B.V.版权所有
We cloned the cDNA of the ecdysone receptor (EcR) isoform-A from the mind bug, Apolygus lucorum (AlEcR-A). The AlEcR-A cDNA has an open reading frame of 1410 bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its A/B-specific domain. Phylogenetic analysis showed that AlEcR-A is very similar to the EcR-A genes of other Hemiptera species. AlEcR-A mRNA was detected at all developmental stages of A. lucorum with peaks correlating to ecdysteroid pulses. AlEcR-A was also expressed in all analyzed tissues with maximum expression in the epidermis and fat body. An AlEcR-A mRNA of size 1.8 kb was detected in all tissues by northern blot analysis. We investigated the functions of AlEcR-A in A. lucorum growth and development using RNAi in vivo. Weights of fifth instar nymphs were significantly decreased in insects treated with AlEcR-A specific anti-sense RNA. Mortality from third instar nymphs to adults increased significantly along with a significant increase in instar duration. (C) 2015 Elsevier B.V. All rights reserved.