EFFECTS OF ACUTE STARVATION ON PAROTID ACINAR CELLS - ULTRASTRUCTURAL AND CYTOCHEMICAL OBSERVATIONS ON AD-LIBITUM-FED AND STARVED RATS
EFFECTS OF ACUTE STARVATION ON PAROTID ACINAR CELLS - ULTRASTRUCTURAL AND CYTOCHEMICAL OBSERVATIONS ON AD-LIBITUM-FED AND STARVED RATS
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DOI：
10.1002/aja.1001350107
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发表时间：
1972-01-01
影响因子：
--
通讯作者：
HAND, AR
中科院分区：
文献类型：
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作者：
HAND, AR
用电镜和细胞化学技术研究了自由进食和饥饿大鼠的腮腺腺泡细胞。在自由进食和饥饿大鼠的细胞中均存在含有酸性磷酸酶和非特异性酯酶活性的溶酶体。它们包括脂褐素样小体、较小的圆形或不规则形状小体、多泡小体和包被囊泡。饥饿16-24小时后，脂滴在基底细胞质中积累，分泌颗粒出现退化迹象。这些改变的颗粒由密度较低基质中的不规则致密物质团块组成。饥饿48-72小时后，改变的颗粒数量增加，并融合形成大的聚集体。一些聚集体还含有囊泡、膜状物质和溶酶体残基。改变的颗粒和聚集体对酸性磷酸酶和非特异性酯酶具有反应性。改变的颗粒的形成似乎是通过分泌颗粒的自发变性发生的，与预先存在的溶酶体和其他改变的颗粒发生继发性融合。结果表明，腮腺腺泡细胞的溶酶体系统的功能，分离和消化分泌颗粒期间减少分泌刺激。
Parotid acinar cells fromad libitum‐fedand starved rats were studied using electron microscopic and cytochemical techniques. Lysosomes containing acid phosphatase and non‐specific esterase activity were present in cells of bothad libitum‐fedand starved rats. They included lipofuscin‐like bodies, smaller round or irregularly‐shaped bodies, multivesicular bodies, and coated vesicles. After 16–24 hours of starvation, lipid droplets had accumulated in the basal cytoplasm, and secretory granules showed evidence of degeneration. These altered granules consisted of irregular clumps of dense material in a less dense matrix. After 48–72 hours of starvation, the altered granules increased in number and fused to form large aggregates. Some of the aggregates also contained vesicles, membranous material, and lysosomal residues. The altered granules and aggregates were reactive for acid phosphatase and non‐specific esterase. The formation of the altered granules appeared to occur by spontaneous degeneration of the secretory granules, with secondary fusion with pre‐existing lysosomes and other altered granules. The results suggest that the lysosomal system of the parotid acinar cell functions to segregate and digest secretory granules during periods of reduced secretory stimulation.