Discriminant function based on hyaluronic acid and its degrading enzymes and degradation products for differentiating cirrhotic from non-cirrhotic liver diseased patients in chronic HCV infection
Discriminant function based on hyaluronic acid and its degrading enzymes and degradation products for differentiating cirrhotic from non-cirrhotic liver diseased patients in chronic HCV infection
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DOI：
10.1016/j.cca.2006.01.004
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发表时间：
2006-07-15
影响因子：
5
通讯作者：
Shiha, GE
中科院分区：
文献类型：
--
作者：
Attallah, AM;Toson, ESA;Shiha, GE
背景/目的：侵入性肝活检仍然被认为是评估慢性丙型肝炎（CHC）患者的金标准。我们的目的是确定一个非侵入性的指标的基础上预测肝硬化在CHC patients.Methods：透明质酸水平测定放射免疫测定法和其降解酶和降解产物的操作特点，在153例CHC与肝硬化和不。结果：多变量判别分析（MDA）选择一个基于5项生化指标绝对值的函数;评分= [1.63 +透明质酸（μ g/l）x 0.001+ N-乙酰基-β-D-氨基葡萄糖苷酶（μ mol/ml/min）× 0.02+葡萄糖醛酸（μ g/dl）× 0.015+葡糖胺（μ g/dl）× 0.006+AST/ALT比值× 0.04]。选择的MDA功能正确分类96%的肝硬化患者，判别临界值=2.5（即小于2.5表示CHC无肝硬化，大于2.5表示肝硬化），具有高度的敏感性（95%）和特异性（97%）。阳性预测值和阴性预测值也很高（分别为95%和97%.Conclusion：CHC患者MDA评分可以简单有效地将其分为急性或非急性肝病患者。(c)2006年由Elsevier B. V.出版
Background/aims: The invasive liver biopsy is still considered the gold standard for assessing patients with chronic hepatitis C (CHC). Our aim was to determine the operating characteristics of a non-invasive index based on blood biomarkers for the prediction of cirrhosis in CHC patients.Methods: Hyaluronic acid level was determined by radioimmuno-assay and its degrading enzymes and degradation products were determined by standard techniques in 153 patients with CHC with and without liver cirrhosis. Statistical analyses were performed by logistic regression, and receiver-operating characteristic (ROC) curves.Results: The multivariate discriminant analysis (MDA) selected a function based on absolute values of five biochemical markers; Score= [1.63 +Hyaluronic acid (mu g/l)x 0.001+N-acetyl-beta-D-glucosaminidase (mu mol/ml/min) x 0.02+glucuronic acid (mu g/dl)x 0.015+ glucosamine (mu g/dl) x 0.006+AST/ALT ratio x 0.04]. The selected MDA function correctly classified 96% of the cirrhotic patients at a discriminant cut-off score=2.5 (i.e. less than 2.5 indicated CHC without liver cirrhosis and greater than 2.5 indicated liver cirrhosis) with high degrees of sensitivity (95%) and specificity (97%). The positive predictive and negative predictive values were also high (95% and 97%, respectively).Conclusion: A patient with CHC can be simply and efficiently classified into cirrhotic or non-cirrhotic liver diseased patient using his or her MDA score. (c) 2006 Published by Elsevier B.V.