Mitochondrial genome of Abraxas suspecta (Lepidoptera: Geometridae) and comparative analysis with other Lepidopterans
Mitochondrial genome of Abraxas suspecta (Lepidoptera: Geometridae) and comparative analysis with other Lepidopterans
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DOI：
10.11646/zootaxa.4254.5.1
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发表时间：
2017-04-20
期刊：
影响因子：
0.9
通讯作者：
Liu, Chao-Liang
中科院分区：
文献类型：
--
作者：
Sun, Yu;Zhang, Jiawei;Liu, Chao-Liang
本文报道了一个完整的拟尺蛾线粒体基因组序列。该基因全长15,547 bp，包含2个核糖体RNA基因、23个转运RNA（tRNA）基因（包括一个额外的tRNA（Asn）（AUU））、13个蛋白质编码基因和一个腺嘌呤（A）+胸腺嘧啶（T）富集区。核苷酸组成和基因组织与其他鳞翅目昆虫相同，除了存在额外的trnN（AUU）拷贝。在38个基因中，25个基因（9个PCG和16个tRNA）由重链（H链）编码，而13个由轻链（L链）编码。在13个PCG中，12个PCG以ATN为起始密码子，而细胞色素c氧化酶亚基1（cox 1）以CGA为起始密码子。13个PCG中的4个具有不完全终止密码子T，而其余的以典型终止密码子终止。所有的tRNA基因都折叠成典型的三叶草结构的线粒体tRNA，除了tRNA（Ser）（AGN）基因，其中DHU臂未能形成稳定的茎环结构。A+ T富集区长532 bp，包含一些保守区域，包括“ATAGA”基序，随后是17 bp的poly-T片段，微卫星样元件（AT）（8）（AAT）（3）和poly-A元件。利用最大似然法（ML）和贝叶斯推理（BI）对13个PCG进行系统发育分析，发现A.嫌疑人居住在Geometridae家庭。本文介绍了利用蛾类作为模式生物进行遗传和进化生物学研究的方法和途径。
In this study, a complete mitochondrial genome (mitogenome) sequence of Abraxas suspecta (Lepidoptera: Geometridae) is isolated and characterized. The complete DNA is 15,547 bp length and contains 2 ribosomal RNA genes, 23 putative transfer RNA (tRNA) genes including an extra tRNA(Asn) (AUU), 13 protein-coding genes and an adenine (A) + thymine (T)-rich region. The nucleotide composition and gene organization are identical to those of other lepidopteran, except for the presence of an extra copy of trnN (AUU). Of the 38 genes, twenty-five genes (9 PCGs and 16 tRNAs) are encoded by heavy strand (H-strand), while thirteen are encoded by light strand (L-strand). Among the 13 PCGs, 12 PCGs employ ATN as initiation codon, while cytochrome c oxidase subunit 1 (cox1) utilizes CGA as initiation codon. Four of the 13 PCGs have the incomplete termination codon T, while the remainder terminated with the canonical stop codon. All tRNA genes are folded into the typical clover-leaf structure of mitochondrial tRNAs, except for the tRNA(Ser) (AGN) gene, in which the DHU arm fails to form a stable stem-loop structure. The A+T-rich region is 532 bp long, and contains some conserved regions, including 'ATAGA' motif followed by a 17bp poly-T stretch, a microsatellite-like element (AT)(8) (AAT)(3) and also a poly-A element. A short Phylogenetic analysis based on 13 PCGs using maximum likelihood (ML) and Bayesian inference (BI) revealed that A. suspecta resides in the Geometridae family. We present the method and approach to use moths as model organisms for further genetic and evolutionary biology studies.