The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.
The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.
复制标题
卡波西肉瘤相关疱疹病毒小衣壳蛋白的组装结构域。
DOI：
10.1128/jvi.01430-12
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发表时间：
2012
影响因子：
5.4
通讯作者：
Desai,PrashantJ
中科院分区：
文献类型：
--
作者：
Kreitler,Dale;Capuano,ChristopherM;Henson,BrandonW;Pryce,ErinN;Anacker,Daniel;McCaffery,JMichael;Desai,PrashantJ
当使用重组杆状病毒在昆虫细胞中共表达六种蛋白质时，卡波西肉瘤相关疱疹病毒衣壳的自组装发生;然而，如果从共感染中省略小衣壳蛋白（SCP），则不会发生组装。在这里，我们描绘和确定精确的装配域和装配所需的SCP残基。因此，组装结构域中的六个残基R14、D18、V25、R46、G66和R70在变为丙氨酸时完全消除或减少衣壳组装。
Self-assembly of Kaposi's sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly.