Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data
Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data
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DOI：
10.1093/jac/dkr289
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发表时间：
2011-10-01
影响因子：
5.2
通讯作者：
Perreten, Vincent
中科院分区：
文献类型：
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作者：
Endimiani, Andrea;Hujer, Kristine M.;Perreten, Vincent
目的：我们调查了是否兽医来源的鲍氏不动杆菌分离株与人类中描述的鲍氏不动杆菌具有共同的分子特征。分析在宠物和马中收集的鲍曼不动杆菌分离株。采用重复基因外回文PCR（rep-PCR）和多位点序列分型（MLST）研究克隆性。结果：rep-PCR扩增出2个主要克隆[A（n=8）和B（n=9）]。MLST表明克隆A含有序列类型（ST）ST 12（国际克隆II）的分离株，克隆B含有ST 15（国际克隆I）的分离株。还观察到两株ST 10和ST 20分离株。17株对庆大霉素耐药，12株对环丙沙星耐药，3株对碳青霉烯类耐药。ST 12的分离株携带bla（OXA-66）、bla（ADC-25）、bla（TEM-1）、aacC 2和IS 1133。ST 15菌株具有bla（OXA-69）、bla（ADC-11）、bla（TEM-1）和携带aacC 1和aadA 1的1类整合子。ISAba 1位于bla（ADC）（1个ST 10和1个ST 12）和/或bla（OXA-66）（7个ST 12）的上游。12个不同ST的分离物含有GyrA中的Ser 83 Leu和ParC中的Ser 80 Leu或Glu 84 Lys替换。未观察到CarO孔蛋白和过表达外排泵的显著破坏。大多数感染是医院获得性的，并在动物的易感条件infection.Conclusions：ST和分子背景的阻力，在我们的集合中观察到的已被频繁地描述在A。在人类患者中检测到鲍曼不动杆菌。动物应被视为多重耐药A的潜在储存库。鲍曼不动杆菌。
Objectives: We investigated whether Acinetobacter baumannii isolates of veterinary origin shared common molecular characteristics with those described in humans.Methods: Nineteen A. baumannii isolates collected in pets and horses were analysed. Clonality was studied using repetitive extragenic palindromic PCR (rep-PCR) and multilocus sequence typing (MLST). PCR and DNA sequencing for various beta-lactamase, aminoglycoside-modifying enzyme, gyrA and parC, ISAba1 and IS1133, adeR and adeS of the AdeABC efflux pump, carO porin and class 1/2/3 integron genes were performed.Results: Two main clones [A (n=8) and B (n=9)] were observed by rep-PCR. MLST indicated that clone A contained isolates of sequence type (ST) ST12 (international clone II) and clone B contained isolates of ST15 (international clone I). Two isolates of ST10 and ST20 were also noted. Seventeen isolates were resistant to gentamicin, 12 to ciprofloxacin and 3 to carbapenems. Isolates of ST12 carried bla(OXA-66), bla(ADC-25), bla(TEM-1), aacC2 and IS1133. Strains of ST15 possessed bla(OXA-69), bla(ADC-11), bla(TEM-1) and a class 1 integron carrying aacC1 and aadA1. ISAba1 was found upstream of bla(ADC) (one ST10 and one ST12) and/or bla(OXA-66) (seven ST12). Twelve isolates of different STs contained the substitutions Ser83Leu in GyrA and Ser80Leu or Glu84Lys in ParC. Significant disruptions of CarO porin and overexpressed efflux pumps were not observed. The majority of infections were hospital acquired and in animals with predisposing conditions for infection.Conclusions: STs and the molecular background of resistance observed in our collection have been frequently described in A. baumannii detected in human patients. Animals should be considered as a potential reservoir of multidrug-resistant A. baumannii.