Quantitative Evaluation of Spermatogenesis by Histochemistry
Quantitative Evaluation of Spermatogenesis by Histochemistry
复制标题
组织化学对精子发生的定量评估
DOI：
10.1007/s00418-022-02080-6
复制
发表时间：
2022
影响因子：
2.3
通讯作者：
Sonoda K Wanta A
中科院分区：
文献类型：
--
作者：
Wakayama T;Yokota S;Noguchi K;Sugawara T;Sonoda K Wanta A
鉴定构成生精小管的生精细胞类型，以及定性确认是否存在疾病，被认为对于精子发生至关重要。精子数量和受精能力都取决于精子发生的质量和数量，是受精的关键因素。然而，精子发生的定量评估并不常见。精子发生具有物种特异性阶段；当精确确定生精小管的特定阶段时，可以自发地识别每个阶段的生精细胞类型。此后，使用独特的标记对每个阶段观察到的细胞进行分类。精子发生的定量评估有可能检测不明显的精子发生障碍或以数字方式指示障碍的程度。为此，使用独特标记的组织化学方法对于精子发生的定量评估是必不可少的。使用计算机软件测量细胞群的技术的未来发展将进一步促进建立精子发生的定量评估作为标准分析方法，这可以极大地促进我们对精子发生的理解。
Identifying the types of spermatogenic cells that compose seminiferous tubules, as well as qualitative confirmation of the presence or absence of disorders, has been regarded as crucial in spermatogenesis. Sperm count and fertilizing capacity, both of which depend on the quality as well as quantity of spermatogenesis, are factors critical to fertilization. However, the quantitative assessment of spermatogenesis is not commonly practiced. Spermatogenesis has species-specific stages; when the specific stage in the seminiferous tubules is precisely determined, the types of spermatogenic cells in each stage can be spontaneously identified. Thereafter, a unique marker is used to classify the cells observed in each stage. Quantitative assessment of spermatogenesis has the potential to detect inapparent spermatogenesis disorders or numerically indicate the degree of the disorder. To this end, a histochemical approach using unique markers is indispensable for the quantitative assessment of spermatogenesis. Future developments in techniques to measure cell populations using computer software will further facilitate the establishment of quantitative assessment of spermatogenesis as a standard analysis method that can contribute significantly to advance our understanding of spermatogenesis.