Sequence conservation and antibody cross-recognition of clade B human immunodeficiency virus (HIV) type 1 Tat protein in HIV-1-infected Italians, Ugandans, and South Africans
Sequence conservation and antibody cross-recognition of clade B human immunodeficiency virus (HIV) type 1 Tat protein in HIV-1-infected Italians, Ugandans, and South Africans
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DOI：
10.1086/378412
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发表时间：
2003-10-15
影响因子：
6.4
通讯作者：
Ensoli, B
中科院分区：
文献类型：
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作者：
Buttò, S;Fiorelli, V;Ensoli, B
我们确定了免疫交叉识别和达特保守的程度，感染当地的人类免疫缺陷病毒（HIV）1型菌株的患者。数据表明，在578份来自HIV感染的意大利（n = 302）、乌干达（n = 139）和南非（n = 137）受试者的血清样本中，总抗Tat IgG和表位特异性抗Tat IgG的流行率相似，使用的是疫苗试验中使用的相同B进化枝达特蛋白。特别是，在来自意大利、乌干达和南非的HIV- 1感染者中分别检测到13.2%、10.8%和13.9%的抗Tat抗体。序列分析结果表明，不同流行病毒的达特与BH-10达特具有高度的相似性，尤其是在1-58个氨基酸区域，该区域包含了大部分的免疫原性表位。这些数据表明，由于达特表位的高度相似性，感染不同本地病毒的个体对B进化枝实验室菌株衍生的达特蛋白疫苗的有效交叉识别。
We determined immune cross-recognition and the degree of Tat conservation in patients infected by local human immunodeficiency virus (HIV) type 1 strains. The data indicated a similar prevalence of total and epitope-specific anti-Tat IgG in 578 serum samples from HIV-infected Italian (n = 302), Ugandan (n = 139), and South African (n = 137) subjects, using the same B clade Tat protein that is being used in vaccine trials. In particular, anti-Tat antibodies were detected in 13.2%, 10.8%, and 13.9% of HIV- 1 - infected individuals from Italy, Uganda, and South Africa, respectively. Sequence analysis results indicated a high similarity of Tat from the different circulating viruses with BH-10 Tat, particularly in the 1-58 amino acid region, which contains most of the immunogenic epitopes. These data indicate an effective cross-recognition of a B-clade laboratory strain-derived Tat protein vaccine by individuals infected with different local viruses, owing to the high similarity of Tat epitopes.