Biohydrogen production improvement using hot compressed water pretreatment on sake brewery waste
Biohydrogen production improvement using hot compressed water pretreatment on sake brewery waste
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DOI：
10.1016/j.ijhydene.2020.04.199
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发表时间：
2020-07
影响因子：
7.2
通讯作者：
M. Choiron;S. Tojo;T. Chosa
中科院分区：
文献类型：
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作者：
M. Choiron;S. Tojo;T. Chosa
大多数氢来自化石燃料。因此，已经研究了更环境友好的制氢方法。本报告描述了热压缩水（HCW）预处理，以提高氢的生产使用清酒酒糟，这是一种工业废物的清酒生产。接种物从处理过的沼液中获得。HCW的温度为130 ° C（0.3MPa）、150 ° C（0.5MPa）和180 °C（0.8MPa），持续15-120分钟。使用气相色谱法分析气体产生;使用HPLC进行发酵液分析。使用改进的Gompertz模型来确定氢势、滞后时间和生产速率。结果表明，随着保持时间的延长和温度的升高，清酒酒糟的降解增加。总糖和有机酸也受到HCW预处理的影响。在130 °C下反应60 min获得最大氢气产率，结果为112.07 mL H2/g COD。HCW预处理成功地缩短了生物制氢的滞后期，提高了酸化程度。丙酮丁醇梭菌（Clostridiumsp.）在所有样品中均检测到成团泛菌，而在2个样品中检测到成团泛菌。
Most hydrogen is derived from fossil fuels. Therefore, more environmentally friendly methods for hydrogen production have been investigated. The present report describes a hot compressed water (HCW) pretreatment to increase hydrogen production using sake lees, which is an industrial waste of sake production. The inoculum is obtained from treated biogas slurry. The temperatures of HCW are 130 (0.3 MPa), 150 (0.5 MPa), and 180 °C (0.8 MPa) for 15–120 min. Gas production was analyzed using gas chromatography; fermentation liquid analyses were performed using HPLC. The modified Gompertz model was used to determine hydrogen potential, lag time, and production rate. Results show an increase in the degradation of sake lees with longer holding time and higher temperature. Total sugar and organic acids also are influenced by HCW pretreatment. The maximum hydrogen yield was obtained at 130 °C for 60 min with the result 112.07 mL H2/g COD. The HCW pretreatment successfully decreased the lag phase of biohydrogen production and increased the degree of acidification.Clostridium butyricum, C. acetobutylicum, and otherClostridiumsp. were identified in all samples, whilePantoea agglomeranswas detected in two samples.