Place cell assemblies remain intact, despite reduced phase precession, after cholinergic disruption.
Place cell assemblies remain intact, despite reduced phase precession, after cholinergic disruption.
复制标题
尽管胆碱能破坏后相位进动减少，但位置细胞组件仍保持完整。
DOI：
10.1002/hipo.23100
复制
发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Newman,EhrenL
中科院分区：
文献类型：
--
作者：
Venditto,SarahJoC;Le,Brianna;Newman,EhrenL
海马θ节律经常被视为一种时钟机制，它协调海马神经元的尖峰活动，形成连贯的神经组件。相位进动是时间编码的一种形式，证明了这种机制，并且在胆碱能信号传导的全身药理破坏后被降解。然而，神经组件是否会相应退化（如时钟机制假设所预测的那样）仍然未知。为了解决这个问题，我们记录了大鼠在全身毒蕈碱乙酰胆碱受体拮抗剂影响之前和期间在巧克力饮料的环形轨道上完成一圈时海马位置细胞的尖峰活动。我们使用三种方法比较了海马体整体的完整性。第一种方法使用交叉相关图（CCG）分析来询问细胞对之间的相对尖峰时间是否变得不太可靠。第二个使用基于一般线性模型的分析来询问同时记录的神经元的活动是否变得不太能预测单个神经元的尖峰活动。最后，第三种方法使用重建分析来询问种群活动对于动物环境位置的信息是否较少以及 θ 序列是否受损。所有三项分析的结果描绘了一致的情况：系统性胆碱能破坏不会降低装配完整性。这些数据表明位置细胞组装并不依赖于高质量的相位进动。
The hippocampal theta rhythm is frequently viewed as a clocking mechanism that coordinates the spiking activity of neurons across the hippocampus to form coherent neural assemblies. Phase precession is a form of temporal coding evidencing this mechanism and is degraded following systemic pharmacological disruption of cholinergic signaling. However, whether neural assemblies are commensurately degraded, as would be predicted from a clocking mechanism hypothesis, remains unknown. To address this, we recorded the spiking activity of hippocampal place cells as rats completed laps on a circle track for chocolate drink before versus during the influence of a systemic muscarinic acetylcholine receptor antagonist. We compared the integrity of hippocampal ensembles using three approaches. The first approach used cross‐correlogram (CCG) analyses to ask if the relative spike‐timing between pairs of cells became less reliable. The second used a general linear model based analysis to ask whether the activity of simultaneously recorded neurons became any less predictive of the spiking activity of single neurons. Finally, the third approach used a reconstruction analysis to ask if the population activity was any less informative regarding the environmental position of the animal and whether theta sequences were impaired. The results of all three analyses paint a consistent picture: systemic cholinergic disruption did not degrade assembly integrity. These data demonstrate that place cell assemblies do not depend upon high quality phase precession.