A Babesia bovis gene syntenic to Theileria parva p67 is expressed in blood and tick stage parasites
A Babesia bovis gene syntenic to Theileria parva p67 is expressed in blood and tick stage parasites
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DOI：
10.1016/j.vetpar.2010.06.024
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发表时间：
2010-10-29
影响因子：
2.6
通讯作者：
Knowles, Donald P.
中科院分区：
文献类型：
--
作者：
Freeman, Jeanne M.;Kappmeyer, Lowell S.;Knowles, Donald P.
完成的Bababelovis（T2 Bo株）基因组提供了详细的数据，预测蛋白质组的这种寄生虫，并允许生物信息学方法的基因发现。血原虫寄生虫B的比较基因组学。牛泰勒虫和小泰勒虫的p67基因侧翼存在一个高度保守的同线基因组。parva。针对东海岸热（ECF）的子孢子阶段特异性疫苗候选物。B中的同线基因。命名为bov 57的bovis编码与p67具有有限氨基酸序列同一性（11.8%）的蛋白质。产生针对重组BOV 57的单克隆抗体，并用于证明BOV 57在B的T2 Bo菌株的裂殖子和动子阶段中的表达。牛。与msa-1（一种先前鉴定的编码红细胞阶段表面蛋白的基因）相比，动粒中bov 57的转录水平增加了100倍。T2 Bo毒株与来自阿根廷和澳大利亚的两种减毒株和强毒株之间的氨基酸序列比较显示，在这些地理上和病原学上不同的分离株中，BOV 57具有高度的序列保守性（97%的氨基酸序列同一性）。另外的基因组比较显示bov 57基因位点在二联巴伯螨和马巴伯螨中也是保守的。虽然不能通过氨基酸或核苷酸序列相似性来识别，但在多个piroplastin中该基因座内的保守基因顺序可能表明适应每个物种独特宿主和生命周期的关键功能。由爱思唯尔公司出版
Completion of the Babesia bovis (T2Bo strain) genome provides detailed data concerning the predicted proteome of this parasite, and allows for a bioinformatics approach to gene discovery. Comparative genomics of the hemoprotozoan parasites B. bovis and Theileria parva revealed a highly conserved syntenic block of genes flanking the p67 gene of T. parva. a sporozoite stage-specific vaccine candidate against East Coast fever (ECF). The syntenic gene in B. bovis, designated bov57, encodes a protein of limited amino acid sequence identity (11.8%) to p67. Monoclonal antibodies were produced against recombinant BOV57 and were used to demonstrate expression of BOV57 in merozoite and kinete stages of the T2Bo strain of B. bovis. Transcript levels of bov57 in kinetes were increased 100-fold in comparison to msa-1, a previously identified gene encoding an erythrocyte stage surface protein. Amino acid sequence comparisons between the T2Bo strain and two attenuated and virulent strains from Argentina and Australia revealed a high degree of sequence conservation in BOV57 among these geographically and pathogenically divergent isolates (97% amino acid sequence identity). Additional genomic comparisons show that the bov57 gene locus is also conserved in Babesia bigemina and Babesia equi. While not identifiable through amino acid or nucleotide sequence similarity, the conserved gene order within this locus in multiple piroplasms may suggest a critical function adapted for each species' unique host and life-cycle. Published by Elsevier B.V.