Expression of cold-inducible RNA-binding protein (CIRP) in pituitary adenoma and its relationships with tumor recurrence.
Expression of cold-inducible RNA-binding protein (CIRP) in pituitary adenoma and its relationships with tumor recurrence.
复制标题
垂体腺瘤中可冷诱导的RNA结合蛋白（CIRP）的表达及其与肿瘤复发的关系。
DOI：
10.12659/msm.893128
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发表时间：
2015-05-02
期刊：
影响因子：
--
通讯作者：
Sun A
中科院分区：
文献类型：
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作者：
Wang M;Zhang H;Heng X;Pang Q;Sun A
本研究的目的是检测冷诱导RNA结合蛋白在垂体腺瘤中的表达，并探讨其对垂体腺瘤复发的影响。我们共收集了60例垂体腺瘤患者的术后样本（其中侵袭性垂体腺瘤20例，非侵袭性腺瘤20例，非侵袭性复发性腺瘤20例）。分别采用Western blotting和real-time PCR检测3种垂体腺瘤组织中CIRP的蛋白和mRNA水平。Western blotting显示侵袭性垂体腺瘤中CIRP表达水平显著高于非侵袭性垂体腺瘤，具有统计学意义（p<0.05）。复发性垂体腺瘤表达的CIRP水平显著高于非复发性肿瘤（p<0.05）。CIRP mRNA的实时PCR获得了一致的结果：侵袭性垂体腺瘤中的转录水平显著高于非侵袭性腺瘤（p<0.05）;复发性腺瘤也具有显著高于非复发性肿瘤的CIRP mRNA水平（p<0.05）。在所有3种类型的垂体腺瘤中，复发肿瘤的CIRP mRNA和蛋白水平最高。CIRP在垂体腺瘤中的表达与肿瘤的增殖和侵袭密切相关，其表达水平显著升高提示术后复发。
The aim of this study was to detect the expression of cold-inducible RNA-binding protein in pituitary adenoma and to determine its effects on tumor recurrence. We collected a total of 60 post-op samples collected from pituitary adenoma patients (including 20 cases of invasive pituitary adenoma, 20 cases of non-invasive adenoma, and 20 cases of non-invasive recurrent adenoma) admitted in our hospital. Both protein and mRNA levels of CIRP in 3 types of pituitary adenoma samples were quantified by Western blotting and real-time PCR, respectively. Western blotting revealed significantly elevated CIRP expression levels in invasive pituitary adenoma compared to non-invasive tumors, with statistical significance (p<0.05). Recurrent pituitary adenoma expressed significantly higher CIRP levels compared to non-recurrent tumors (p<0.05). Real-time PCR for CIRP mRNA obtained consistent results: transcript levels were significantly higher in invasive pituitary adenoma compared to non-invasive adenoma (p<0.05); recurrent adenoma also had significantly higher CIRP mRNA levels compared to non-recurrent tumors (p<0.05). Among all 3 types of pituitary adenoma, recurrent tumors had the highest levels of CIRP mRNA and protein. The expression of CIRP in pituitary adenoma is closely related with tumor proliferation and invasion, and its significantly elevated expression level indicates post-op recurrence.