Effect of electroacupuncture on visceral hyperalgesia, serotonin and fos expression in an animal model of irritable bowel syndrome.
Effect of electroacupuncture on visceral hyperalgesia, serotonin and fos expression in an animal model of irritable bowel syndrome.
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DOI：
10.5056/jnm.2010.16.3.306
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发表时间：
2010-07
影响因子：
3.4
通讯作者：
Sung JJ
中科院分区：
文献类型：
--
作者：
Wu JC;Ziea ET;Lao L;Lam EF;Chan CS;Liang AY;Chu SL;Yew DT;Berman BM;Sung JJ
虽然针灸可以缓解躯体疼痛，但其对内脏疼痛如肠易激综合征（IBS）的治疗效果尚不清楚。我们在动物模型上评价了针刺治疗内脏痛觉过敏的效果。Sprague-Dawley大鼠（n = 8/组）与先前的新生儿母亲分离的压力被随机分配到接受3天的治疗，无论是电针（EA）或假针刺穴位ST-36。另一组未进行母体分离的大鼠作为未处理对照。在针刺治疗后的第二天进行结直肠扩张。比较3组动物的腹缩反射痛阈和肌电图内脏反应。取结肠、脊髓和脑干组织，用免疫组织化学方法进行5-羟色胺和Fos表达的地形分布和定量评估。EA组大鼠的痛阈明显高于假针刺组（25.0 ± 5.7 mmHg vs 18.7 ± 5.2 mmHg，p = 0.01），与未处理的未处理对照组（29.4 ± 9.3 mmHg，p = 0.28）相当。在所有结直肠扩张压力下，与接受假针刺的患者相比，通过肌电图测量，他们的内脏反应也较低。电针可显著抑制脑干中缝背核、脊髓背角浅层和结肠上皮的Fos表达，但仅抑制脑干和脊髓的5-HT表达。电针通过下调脑-肠轴的中枢神经元活动减轻内脏痛觉过敏。
While it is well established that acupuncture relieves somatic pain, its therapeutic effect on visceral pain such as irritable bowel syndrome (IBS) is unclear. We evaluated the effect of acupuncture in treating visceral hyperalgesia in an animal model. Sprague-Dawley rats (n = 8 per group) with prior neonatal maternal separation stress were randomly allocated to receive 3-day treatment of either electroacupuncture (EA) or sham acupuncture at acupoint ST-36. Another group of rats without prior maternal separation was included as non-handled controls. Colorectal distension was performed on the day after acupuncture treatment. The 3 groups were compared for pain threshold as determined by abdominal withdrawal reflex and visceromotor response as measured by electromyogram. Colon, spinal cord, and brainstem were sampled for topographic distribution and quantitative assessment of serotonin and Fos expression by immunohistochemistry. Rats in EA group had significantly higher pain threshold compared to those in sham acpuncture group (25.0 ± 5.7 mmHg vs 18.7 ± 5.2 mmHg, p = 0.01) and it was comparable with that of non-handled treatment naïve controls (29.4 ± 9.3 mmHg, p = 0.28). They also had lower visceromotor response as measured by electromyogram compared to those received sham acupuncture at all colorectal distension pressures. EA significantly suppressed Fos expression in doral raphe nuclei of brainstem, superficial dorsal horn of spinal cord and colonic epithelium but suppressed 5-HT expression only in brainstem and spinal cord. Electro acupuncture attenuates visceral hyperlagesia through down-regulation of central serotonergic activities in the brain-gut axis.