Distinct substance P- and calretinin-containing projections from the supramammillary area to the hippocampus in rats; A species difference between rats and monkeys
Distinct substance P- and calretinin-containing projections from the supramammillary area to the hippocampus in rats; A species difference between rats and monkeys
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DOI：
10.1007/pl00005706
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发表时间：
1997-06-01
影响因子：
2
通讯作者：
Leranth, C
中科院分区：
文献类型：
--
作者：
Borhegyi, Z;Leranth, C
我们最近的研究表明，在猴的乳头上-海马通路中，P物质和钙视黄蛋白共存，以及猴和大鼠的海马中外源性P物质纤维的突触靶点存在种属差异。使用的实验：（1）将麦胚凝集素结合的HRP单次和多次立体定位注射到海马内，并在乳头体上区进行P物质的免疫染色;（2）P物质和钙维生素A在乳头体上区细胞中的共定位;（3）这两种神经化学物质在逆行标记的雄性和雌性大鼠乳头体上投射细胞中的共定位。这表明：（a）在同侧乳头体上区有许多钙视黄蛋白和较少的P物质免疫反应神经元逆行标记;（B）大约74%的P物质细胞含有钙视黄蛋白，9%的钙视黄蛋白神经元含有P物质;并且，在本发明中，最重要的是（c）没有逆行标记的乳头体上细胞共定位钙视网膜蛋白和P物质。这些结果表明存在两种不同的乳头体上细胞，大鼠海马投射，一个含有P物质，另一个含有钙视网膜蛋白。后者支配与猴子相同的区域，前者仅终止于CA 2海马子区。
Our recent studies showed the co-existence of substance P and calretinin in the supramammillo-hippocampal pathway of monkeys, as well as species differences in the synaptic targets of extrinsic substance P fibers in the hippocampi of monkeys and rats. Experiments used: (1) single and multiple stereotaxic injection of wheat germ agglutinin-conjugated HRP into the hippocampus and immunostaining for substance P in the su pramammillary area; (2) colocalization of substance P and calretinin in supramammillary area cells; and (3) colocalization of these two neurochemicals in retrogradely labeled supramammillary projective cells of both male and female rats. These demonstrated: (a) many calretinin- and fewer substance P-immunoreactive neurons retrogradely labeled in the ipsilateral supramammillary area; (b) approximately 74% of all substance P cells contain calretinin and 9% of the calretinin neurons co-contain substance P; and, most importantly (c) none of the retrogradely labeled supramammillary cells colocalize calretinin and substance P. These results indicate the presence of two distinct supramammillo-hippocampal projections in the rat, one that contains substance P and the other calretinin. The latter innervates the same areas as those in the monkey, and the former terminates only in the CA2 hippocampal subfield.