MONOCLONAL-ANTIBODIES AGAINST RAT IMMUNOGLOBULIN KAPPA-CHAINS
MONOCLONAL-ANTIBODIES AGAINST RAT IMMUNOGLOBULIN KAPPA-CHAINS
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DOI：
10.1089/hyb.1.1982.1.125
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
WARNER, NL
中科院分区：
文献类型：
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作者：
LANIER, LL;GUTMAN, GA;WARNER, NL
用可溶性大鼠免疫球蛋白免疫SJL/J小鼠，免疫脾细胞与P3-X63-Ag8骨髓瘤细胞融合，制备了针对大鼠kappa轻链的单抗。这些杂交瘤细胞系中的三个株MAR18.5、80.2和103.6的单抗已被广泛鉴定。MAR18.5、80.2和103.6抗体属于λ2a x亚型，与蛋白A结合强烈，易于纯化。第18.5号克隆的单抗与RL-1a和RL-1b的Ig结合良好，而80.2和103.6的抗体选择性地与RL-1b结合。这些单抗可以被FITC偶联，作为间接免疫荧光分析中的第二抗体，或者标记为用于需要特定抗鼠X抗体的放射免疫分析。抗同种异型特异性的单抗也可用于大鼠免疫球蛋白遗传学的研究。
Monoclonal antibodies directed against rat kappa light chains have been generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by fusion of immune spleen cells with the P3-X63-Ag8 myeloma cell line. Monoclonal antibodies from three of these hybridoma cell lines, MAR 18.5, 80.2, and 103.6, have been extensively characterized. MAR 18.5, 80.2, and 103.6 antibodies are of the λ2a x isotype, and bind strongly to protein A, allowing easy purification. Monoclonal antibody from clone 18.5 binds equally well to Ig of both Rl-la and Rl-lb allotypes, whereas 80.2 and 103.6 antibodies selectively bind to Rl-lb. These monoclonal antibodies can be FITC conjugated for use as a second antibody in indirect immunofluorescence assays, or radiolabeled for use in radio immunoassays requiring a specific antirat x antibody. The antiallotype specific monoclonal antibodies also may be of use in the study of rat immunoglobulin genetics.