Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter
Transient transfection of intraerythrocytic Babesia gibsoni using elongation factor-1 alpha promoter
复制标题
DOI：
10.1016/j.molbiopara.2017.07.003
复制
发表时间：
2017-09-01
影响因子：
1.5
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Liu, Mingming;Asada, Masahito;Xuan, Xuenan
The development of gene manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not yet been established for Babesia gibsoni. Here, we report for the first time, the successful transient transfection of B. gibsoni. The plasmid containing the firefly luciferase reporter gene (pBS-ELA) was transfected into B. gibsoni by an AMAXA 4D Nudeofector (TM) device. Transfection using program FA113 and Lonza buffer SF showed the highest luciferase expression. Twenty micrograms of plasmid produced the highest relative transfection efficiency. The fluorescent protein-expressing parasites were determined by GFP-containing plasmid (pBS-EGA) at 48 and 72 h post transfection. This finding is the first step towards a stable transfection method for B. gibsoni, which may contribute to a better understanding of the biology of the parasite.
