MUREIN HYDROLASES IN ENVELOPE OF ESCHERICHIA-COLI - PROPERTIES IN-SITU AND SOLUBILIZATION FROM ENVELOPE
MUREIN HYDROLASES IN ENVELOPE OF ESCHERICHIA-COLI - PROPERTIES IN-SITU AND SOLUBILIZATION FROM ENVELOPE
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DOI：
10.1111/j.1432-1033.1974.tb03261.x
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
SCHWARZ, U
中科院分区：
文献类型：
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作者：
HARTMANN, R;BOCKHENN.SB;SCHWARZ, U
描述了被认为在细菌形态发生中发挥重要作用的胞壁质水解酶的特性。有证据表明，在完整的细胞中，这些酶可以防止不受控制的反应。研究表明，酶与其底物球囊（细胞膜内的一种保持形状的大分子）之间的“屏障”可以被人为打破。描述了测量完整细胞、分离的细胞包膜中和溶解后的胞壁质水解酶活性的测定系统。 Triton X-100 和氯化钠的组合可有效释放膜结合水解酶。
Properties of murein hydrolases, assumed to play an essential role in bacterial morphogenesis, are described. Evidence is presented that in the intact cell these enzymes are prevented from uncontrolled reaction. It is shown that the “barrier” between the enzymes and their substrate sacculus, a shape‐maintaining macromolecule within the cell envelope, can be artificially broken. Assay systems to measure murein hydrolase activity in the intact cell, in isolated cell envelopes and after solubilization are described. A combination of Triton X‐100 and sodium chloride is shown to liberate membrane‐bound hydrolases effectively.