Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene
Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene
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DOI：
10.1016/j.brainres.2007.05.012
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发表时间：
2007-11-07
期刊：
影响因子：
2.9
通讯作者：
Oka, Shogo
中科院分区：
文献类型：
--
作者：
Inoue, Mitsuhiro;Kato, Keiko;Oka, Shogo
HNK-1碳水化合物表位是位于糖链非还原末端的一种硫化葡萄糖醛酸，表达在糖蛋白和糖脂上，通过影响粘附性和抗粘附性来调节轴突生长和突触可塑性。已知HNK-1碳水化合物的合成是通过两个关键酶--葡萄糖醛酸基转移酶(GlcAT-P和GlcAT-S)来完成的。在本研究中，我们用原位杂交组织化学和免疫组织化学方法研究了GlcAT转录本和HNK-1碳水化合物在有或没有GlcAT-P基因的成年小鼠脑中的定位。观察到两个GICAT转录本的区域特异性表达模式。GICAT-P的强表达和GlcAT-S的中等表达见于边缘相关区域的多个核团、感觉系统和小脑。组织学研究表明，HNK-1碳水化合物的定位与GlcAT转录本在脑中的表达模式平行。此外，HNK-1碳水化合物在GlcAT-P缺陷小鼠脑中的定位仅限于部分，而HNK-1碳水化合物广泛分布于野生型小鼠的大部分大脑中。本研究为理解HNK-1碳水化合物在中枢神经系统中构建的网络提供了一个新的框架。(C)2007 Elsevier B.V.保留所有权利。
The HNK-1 carbohydrate epitope, a sulfated glucuronic acid at the non-reducing terminus of glycans, is expressed on glycoproteins and glycolipids and modulates neurite outgrowth and synaptic plasticity by affecting the adhesive and anti-adhesive properties. It is known that the HNK-1 carbohydrate is synthesized through two key enzymes, glucuronyltransferases (GlcAT-P and GlcAT-S). In the present study, we investigated the localization of GlcAT transcripts and HNK-1 carbohydrate in the adult mouse brain with or without GlcAT-P gene using in situ hybridization histochemistry and immunohistochemistry. Region-specific expression patterns of both GIcAT transcripts were observed. Strong expression of GIcAT-P and moderate expression of GlcAT-S were seen in neuronal cells of several nuclei of limbic-related regions and of the sensory system and the cerebellum. It was shown histologically that the localization of HNK-1 carbohydrate paralleled the pattern of expression of GlcAT transcripts in the brain. Additionally, the localization of HNK-1 carbohydrate was restricted partially in the brain of GlcAT-P -deficient mice, while the HNK-1 carbohydrate was widely distributed over most of the brain of wild-type mice. The present study provides a new framework for understanding the network constructed by the HNK-1 carbohydrate in the central nervous system. (c) 2007 Elsevier B.V. All rights reserved.