Rrp6, Rrp47 AND COFACTORS OF THE NUCLEAR EXOSOME
Rrp6, Rrp47 AND COFACTORS OF THE NUCLEAR EXOSOME
复制标题
DOI：
10.1007/978-1-4419-7841-7_8
复制
发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Mitchell, Phil
中科院分区：
文献类型：
--
作者：
Butler, J. Scott;Mitchell, Phil
本章回顾了在细胞核中与RNA外泌体一起起作用的酶的活性的知识现状。在这个隔室中，外泌体在物理和功能上与外核糖核酸酶Rrp6和几个辅因子相互作用，最突出的是Rrp47和TRAMP复合体。这些相互作用决定了RNA前体的命运，从转录到成熟核糖核蛋白颗粒（RNPs）的形成以及RNPs向细胞质的输出。核外泌体催化许多这些rna的成熟3'端形成，但在其他情况下将rna降解为单核苷酸。辅助因子如Mpp6、TRAMP和Nrd1/Nab3复合物在决定RNPs与核外泌体相互作用的结果中起重要作用。支配这些决定的特殊性的细节仍然是未来调查的丰富来源。
This chapter reviews the present state of knowledge on the activity of enzymes that function with the RNA exosome in the nucleus. In this compartment, the exosome interacts physically and functionally with the exoribonuclease Rrp6 and several cofactors, most prominently Rrp47 and the TRAMP complex. These interactions decide the fate of RNA precursors from transcription through the formation of mature ribonucleoprotein particles (RNPs) and the export of the RNPs to the cytoplasm. The nuclear exosome catalyzes the formation of the mature 3' ends of many of these RNAs, but in other cases degrades the RNAs to mononucleotides. Cofactors such as Mpp6, TRAMP and the Nrd1/Nab3 complex play important roles in determining the outcome of the interaction of RNPs with the nuclear exosome. The details that govern the specificity of these decisions remain a rich source for future investigation.