Sub-second isoelectric focusing in free flow using a microfluidic device
Sub-second isoelectric focusing in free flow using a microfluidic device
复制标题
DOI：
10.1039/b308476k
复制
发表时间：
2003-01-01
期刊：
影响因子：
6.1
通讯作者：
Manz, A
中科院分区：
文献类型：
--
作者：
Xu, Y;Zhang, CX;Manz, A
使用一个微加工芯片的床体积为0.2穆尔，我们证明了标度律的有效性等电聚焦（IEF）在自由流动中的分子质量传输。纳升或微升样品体积可在430 ms内浓缩高达400倍。这些非常快的性能使芯片适用于蛋白质组学分析和连续监测生化过程。
Using a microfabricated chip with a bed volume of 0.2 muL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.