Two-dimensional protein electrophoresis: from molecular pathway discovery to biomarker discovery in neurological disorders.
Two-dimensional protein electrophoresis: from molecular pathway discovery to biomarker discovery in neurological disorders.
复制标题
二维蛋白质电泳：从分子途径发现到神经系统疾病生物标志物发现。
DOI：
10.1016/j.nurx.2006.05.001
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Lee,KelvinH
中科院分区：
文献类型：
--
作者：
Choe,LeilaH;Werner,BrendaG;Lee,KelvinH
在过去的30年里，二维蛋白质电泳(2-DE)经历了许多技术的改进，产生了一种分析方法，其在分辨复杂蛋白质混合物方面是无与伦比的，并且能够量化来自各种组织和样本的蛋白质表达的变化。这项技术已经被应用于许多神经系统疾病的研究，以确定与疾病相关的斑点模式的变化。当它与最先进的质谱学方法相结合时，这项技术的真正力量就显现了出来，这使得能够在2-DE地图上识别一个或多个感兴趣的点内包含的蛋白质。研究人员已经成功地应用了这项技术，以更好地了解人类和动物模型中的神经疾病机制，并发现在临床环境中有用的生物标志物。到目前为止还没有实现的这些努力的一个重要扩展是希望描述治疗导致的蛋白质表达的变化，以帮助在分子水平上将疾病修改效果与临床结果联系起来。在这里，我们回顾了2-DE方法的主要进展，并讨论了它在神经系统疾病研究中的具体应用实例。
Two-dimensional protein electrophoresis (2-DE) has undergone many technical improvements in the past 30 years, resulting in an analytical method that is unparalleled in the resolution of complex protein mixtures and capable of quantifying changes in protein expression from a wide variety of tissues and samples. The technique has been applied in many studies of neurologic disease to identify changes in spot patterns that correlate with disease. The true power of the technique emerges when it is coupled to state-of-the-art methods in mass spectrometry, which enable identification of the protein or proteins contained within a spot of interest on a 2-DE map. Investigators have successfully applied the technique to gain improved understanding of neurologic disease mechanisms in humans and in animal models and to discover biomarkers that are useful in the clinical setting. An important extension to these efforts that has not been realized thus far is the desire to profile changes in protein expression that result from therapy to help relate disease-modifying effects at the molecular level with clinical outcomes. Here we review the major advances in 2-DE methods and discuss specific examples of its application in the study of neurologic diseases.