HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY
HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY
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DOI：
10.1099/00207713-42-1-166
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发表时间：
1992-01-01
期刊：
影响因子：
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通讯作者：
JURTSHUK, P
中科院分区：
文献类型：
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作者：
FOX, GE;WISOTZKEY, JD;JURTSHUK, P
16S rRNA（编码rRNA的基因）序列与三种嗜冷菌株：芽孢杆菌globisporus W25T （T =型菌株）、嗜冷芽孢杆菌W16A(T)和W5进行比较。这些菌株具有99.5%以上的序列同一性，在实验不确定度范围内可视为相同。表型相似性进一步证明了它们密切的分类学关系。相比之下，先前发表的DNA-DNA杂交结果令人信服地确定，如果使用当前的标准，这些菌株不属于同一物种。这些结果强调了重要的一点，即16S rRNA序列的有效识别不一定是保证物种身份的充分标准。因此，尽管16S rRNA序列可以常规地用于区分和建立属与已确定的物种之间的关系，但最近分化的物种可能无法识别。
16S rRNA (genes coding for rRNA) sequence comparisons were conducted with the following three psychrophilic strains: Bacillus globisporus W25T (T = type strain) and Bacillus psychrophilus W16A(T), and W5. These strains exhibited more than 99.5% sequence identity and within experimental uncertainty could be regarded as identical. Their close taxonomic relationship was further documented by phenotypic similarities. In contrast, previously published DNA-DNA hybridization results have convincingly established that these strains do not belong to the same species if current standards are used. These results emphasize the important point that effective identity of 16S rRNA sequences is not necessarily a sufficient criterion to guarantee species identity. Thus, although 16S rRNA sequences can be used routinely to distinguish and establish relationships between genera and well-resolved species, very recently diverged species may not be recognizable.