CHLOROPLAST RIBOSOME BIOGENESIS IN CHLAMYDOMONAS
CHLOROPLAST RIBOSOME BIOGENESIS IN CHLAMYDOMONAS
复制标题
衣藻中叶绿体核糖体的生物发生
DOI：
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发表时间：
1974
影响因子：
7.8
通讯作者：
N. W. Gillham
中科院分区：
文献类型：
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作者：
E. H. Harris;J. Boynton;N. W. Gillham
莱茵衣藻中叶绿体蛋白的合成在提供乙酸作为碳源时是必不可少的。因此，叶绿体核糖体合成、组装或功能缺陷的突变体是有条件存活的。非光合细胞对砷酸盐的正选择与简单的筛选程序相结合，分离出叶绿体蛋白质合成中具有广谱缺陷的突变体。分离出8个叶绿体核糖体缺陷突变体。其中三种已被确定为遗传和表型特征，并与先前描述的两种核糖体突变体ac-20和cr-1进行了比较。本文提出了一个核糖体组装的工作模型，该模型提出了这五个孟德尔基因位点可能的生化作用。
Chloroplast protein synthesis in Chlamydomonas reinhardtii is dispensable when cells are provided acetate as a carbon source. Mutants defective in synthesis, assembly, or function of chloroplast ribosomes are therefore conditionally viable. Positive selection of nonphotosynthetic cells on arsenate has been combined with a simple screening procedure to isolate mutants with a broad spectrum of defects in chloroplast protein synthesis. Eight new mutants deficient in chloroplast ribosomes have been isolated. Three of these have been characterized genetically and phenotypically, and compared with two previously described ribosome mutants, ac-20 and cr-1. A working model of ribosome assembly is proposed which suggests possible biochemical roles for these five Mendelian gene loci.