HIGH DOSE PER-FRACTION IRRADIATION OF LIMITED LUNG VOLUMES USING AN IMAGE-GUIDED, HIGHLY FOCUSED IRRADIATOR: SIMULATING STEREOTACTIC BODY RADIOTHERAPY REGIMENS IN A SMALL-ANIMAL MODEL
HIGH DOSE PER-FRACTION IRRADIATION OF LIMITED LUNG VOLUMES USING AN IMAGE-GUIDED, HIGHLY FOCUSED IRRADIATOR: SIMULATING STEREOTACTIC BODY RADIOTHERAPY REGIMENS IN A SMALL-ANIMAL MODEL
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DOI：
10.1016/j.ijrobp.2009.12.074
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发表时间：
2010-07-01
影响因子：
7
通讯作者：
Story, Michael D.
中科院分区：
文献类型：
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作者：
Cho, Jaeho;Kodym, Reinhard;Story, Michael D.
目的：为了研究与立体定向体部放射治疗（SBRT）相关的潜在生物学，体内模型和图像引导的高聚焦照射系统都是必要的。在这里，我们描述了这样一个照射系统，并使用它来检查正常组织的毒性在一个小动物模型中的肺体积类似于与人类therapy.Methods和材料：高剂量的辐射被传递到一个小体积的C3 H/HeJCr小鼠的左肺使用一个小动物的立体定向辐照器。辐射器具有产生聚焦辐射束的准直机构、由耦合到电荷耦合器件相机的荧光屏组成的成像子系统以及手动定位平台。组织学检查和micro-CT被用来评估辐射respons.Results：局灶性闭塞的肺泡纤维结缔组织，支气管上皮细胞增生，以及存在少量的炎性细胞的小鼠肺的主要反应，低容量/高剂量照射。组织反应表明早期纤维化的辐射剂量阈值在40和100戈伊之间。照射系统满足我们的要求，高剂量率，小束径，精确的定位和verified.Conclusions：我们已经建立了一个实验模型和图像引导的动物照射系统的研究，高剂量每分数照射，如那些用于SBRT在体积类似于那些用于人类。它还将允许靶向胸部的特定解剖结构或最终靶向肺的原位肿瘤。(C)2010年爱思唯尔公司
Purpose: To investigate the underlying biology associated with stereotactic body radiotherapy (SBRT), both in vivo models and image-guided, highly focal irradiation systems are necessary. Here, we describe such an irradiation system and use it to examine normal tissue toxicity in a small-animal model at lung volumes similar to those associated with human therapy.Methods and Materials: High-dose radiation was delivered to a small volume of the left lung of C3H/HeJCr mice using a small-animal stereotactic irradiator. The irradiator has a collimation mechanism to produce focal radiation beams, an imaging subsystem consisting of a fluorescent screen coupled to a charge-coupled device camera, and a manual positioning stage. Histopathologic examination and micro-CT were used to evaluate the radiation response.Results: Focal obliteration of the alveoli by fibrous connective tissue, hyperplasia of the bronchiolar epithelium, and presence of a small number of inflammatory cells are the main reactions to low-volume/high-dose irradiation of the mouse lung. The tissue response suggested a radiation dose threshold for early phase fibrosis lying between 40 and 100 Gy. The irradiation system satisfied our requirements of high-dose-rate, small beam diameter, and precise localization and verification.Conclusions: We have established an experimental model and image-guided animal irradiation system for the study of high dose per fraction irradiations such as those used with SBRT at volumes analogous to those used in human beings. It will also allow the targeting of specific anatomical structures of the thorax or ultimately, orthotopic tumors of the lung. (C) 2010 Elsevier Inc.