Molecular detection and prevalence of enterovirus within environmental water in Taiwan
Molecular detection and prevalence of enterovirus within environmental water in Taiwan
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DOI：
10.1111/j.1365-2672.2007.03598.x
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Wan, M. -T.
中科院分区：
文献类型：
--
作者：
Chen, C. -H.;Hsu, B. -M.;Wan, M. -T.
目的：为了解2004-2005年台湾地区不同环境水样中肠道病毒RNA的存在情况，并分析台湾地区肠道病毒的基因型和分布情况，采用逆转录聚合酶链反应（RT-PCR）技术对131份环境水样进行了肠道病毒5 '非翻译区（5'-nontranslated regions，5 '-NTR）的检测。在这些肠道病毒RNA阳性样品中，分别从地表水、地下水和污水中回收了32、15和6个样品。但应用双套式PCR后，总阳性检出率提高到40.5%。测序结果显示，大多数分离株属于以下基因型：科萨基A2（35.8%），科萨基A6（13.2%）和肠道病毒（EV）71（11.3%）;还观察到埃可病毒11、猪EV 9和科萨基A16分离株。本研究证实肠道病毒的主要流行基因型在台湾的地表水和地下水中普遍存在。这项研究有助于了解该病毒在研究区域内外的意义和流行病学。此外，还可以预测肠道病毒的基因型，并评估水污染与临床样本中发现的病毒序列之间可能的相关性。
Aims: To investigate the presence of enterovirus RNA in various samples of environmental water collected in Taiwan during 2004-2005, and to characterize the genotypes and distribution of the viruses identified in Taiwan.Methods and Results: Total 131 environmental samples were screened using the reverse transcription polymerase chain reaction (RT-PCR) for the highly conserved 5'-nontranslated regions (5'-NTR). Among these enterovirus RNA-positive samples, 32, 15 and 6 of the samples were recovered from surface water, ground water and sewage water respectively. However, the total positive detection rate increased to 40.5% with the application of seminested PCR. Sequencing revealed that the majority of isolates belonged to the following genotypes: coxsackie A2 (35.8%), coxsackie A6 (13.2%) and enterovirus (EV)71 (11.3%); echovirus 11, porcine EV9 and coxsackie A16 isolates were also observed.Conclusions: This study confirms that the major epidemic genotypes of enterovirus are prevalent in the surface and ground water of Taiwan.Significance and Impact of the Study: This study is helpful in understanding the significance and epidemiology of the virus within and beyond the study area. Moreover, it was possible to predict the enterovirus genotype and evaluate possible correlations between water contamination and viral sequences found among clinical samples.